基于羧甲基纤维素的支架与微小RNA-15b对成骨细胞分化的联合作用
A Combinatorial effect of carboxymethyl cellulose based scaffold and microRNA-15b on osteoblast differentiation.
作者信息
Vimalraj S, Saravanan S, Vairamani M, Gopalakrishnan C, Sastry T P, Selvamurugan N
机构信息
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
Nanotechnology Research Center, SRM University, Kattankulathur, Tamil Nadu, India.
出版信息
Int J Biol Macromol. 2016 Dec;93(Pt B):1457-1464. doi: 10.1016/j.ijbiomac.2015.12.083. Epub 2016 Jan 2.
The present study was aimed to synthesize and characterize a bio-composite scaffold containing carboxymethyl cellulose (CMC), zinc doped nano-hydroxyapatite (Zn-nHAp) and ascorbic acid (AC) for bone tissue engineering applications. The fabricated bio-composite scaffold was characterized by SEM, FT-IR and XRD analyses. The ability of scaffold along with a bioactive molecule, microRNA-15b (miR-15b) for osteo-differentiation at cellular and molecular levels was determined using mouse mesenchymal stem cells (mMSCs). miR-15b acts as posttranscriptional gene regulator and regulates osteoblast differentiation. The scaffold and miR-15b were able to promote osteoblast differentiation; when these treatments were combined together on mMSCs, there was an additive effect on promotion of osteoblast differentiation. Thus, it appears that the combination of CMC/Zn-nHAp/AC scaffold with miR-15b would provide more efficient strategy for treating bone related defects and bone regeneration.
本研究旨在合成并表征一种用于骨组织工程应用的生物复合支架，该支架包含羧甲基纤维素（CMC）、锌掺杂纳米羟基磷灰石（Zn-nHAp）和抗坏血酸（AC）。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）分析对制备的生物复合支架进行表征。使用小鼠间充质干细胞（mMSCs）测定支架与生物活性分子微小RNA-15b（miR-15b）在细胞和分子水平上促进骨分化的能力。miR-15b作为转录后基因调节因子，调节成骨细胞分化。该支架和miR-15b能够促进成骨细胞分化；当这些处理联合作用于mMSCs时，对促进成骨细胞分化有累加效应。因此，CMC/Zn-nHAp/AC支架与miR-15b的组合似乎将为治疗骨相关缺陷和骨再生提供更有效的策略。
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