壳聚糖/纳米羟基磷灰石/纳米氧化锆二氧化钛支架负载 miR-590-5p 促进骨再生。
Chitosan/nano-hydroxyapatite/nano-zirconium dioxide scaffolds with miR-590-5p for bone regeneration.
机构信息
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.
Centre for Nanotechnology and Advanced Biomaterials, Department of Bioengineering, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, Tamil Nadu, India.
出版信息
Int J Biol Macromol. 2018 May;111:953-958. doi: 10.1016/j.ijbiomac.2018.01.122. Epub 2018 Feb 19.
Bone tissue engineering (BTE) relies on biocomposite scaffolds and bioactive molecules for bone regeneration. The present study was aimed to synthesize and characterize biocomposite scaffolds containing chitosan (CS), nano-hydroxyapatite (nHAp) and nano‑zirconium dioxide (nZrO) along with microRNA (miRNA) for BTE applications. miRNAs act as post-transcriptional regulator of gene expression. The fabricated biocomposite scaffolds were characterized using SEM, FT-IR and XRD analyses. The effect of a bioactive molecule (miR-590-5p) with scaffolds was tested for osteoblast differentiation at the cellular and molecular levels using mouse mesenchymal stem cells (C3H10T1/2). The results showed that CS/nHAp/nZrO scaffolds promoted osteoblast differentiation, and this effect was further increased in the presence of miR-590-5p in C3H10T1/2 cells. Thus, we suggested that CS/nHAp/nZrO scaffolds with miR-590-5p would have potential towards the treatment of bone defects.
骨组织工程（BTE）依赖于生物复合材料支架和生物活性分子来实现骨再生。本研究旨在合成和表征含有壳聚糖（CS）、纳米羟基磷灰石（nHAp）和纳米氧化锆（nZrO）以及 microRNA（miRNA）的生物复合材料支架，用于 BTE 应用。miRNA 作为基因表达的转录后调节剂。使用 SEM、FT-IR 和 XRD 分析对制备的生物复合材料支架进行了表征。使用小鼠间充质干细胞（C3H10T1/2）在细胞和分子水平上测试了具有支架的生物活性分子（miR-590-5p）对成骨细胞分化的影响。结果表明，CS/nHAp/nZrO 支架促进成骨细胞分化，而在 C3H10T1/2 细胞中存在 miR-590-5p 时，这种作用进一步增强。因此，我们认为 CS/nHAp/nZrO 支架与 miR-590-5p 结合具有治疗骨缺损的潜力。
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