Dash Satyakam, Ng Chiam Yu, Maranas Costas D
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802-1503, USA.
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802-1503, USA
FEMS Microbiol Lett. 2016 Feb;363(4). doi: 10.1093/femsle/fnw004. Epub 2016 Jan 10.
Anaerobic Clostridium spp. is an important bioproduction microbial genus that can produce solvents and utilize a broad spectrum of substrates including cellulose and syngas. Genome-scale metabolic (GSM) models are increasingly being put forth for various clostridial strains to explore their respective metabolic capabilities and suitability for various bioconversions. In this study, we have selected representative GSM models for six different clostridia (Clostridium acetobutylicum, C. beijerinckii, C. butyricum, C. cellulolyticum, C. ljungdahlii and C. thermocellum) and performed a detailed model comparison contrasting their metabolic repertoire. We also discuss various applications of these GSM models to guide metabolic engineering interventions as well as assessing cellular physiology.
厌氧梭菌属是一类重要的生物生产微生物，能够产生溶剂，并利用包括纤维素和合成气在内的多种底物。越来越多的基因组尺度代谢（GSM）模型被用于各种梭菌菌株，以探索它们各自的代谢能力以及对各种生物转化的适用性。在本研究中，我们选择了六种不同梭菌（丙酮丁醇梭菌、拜氏梭菌、丁酸梭菌、纤维素分解梭菌、产乙醇梭菌和嗜热栖热梭菌）的代表性GSM模型，并对它们的代谢功能进行了详细的模型比较。我们还讨论了这些GSM模型在指导代谢工程干预以及评估细胞生理学方面的各种应用。
