通过使用亚油酸13-水合酶和全解脂耶氏酵母细胞的一锅法反应，由亚油酸经13-羟基-9(Z)-十八碳烯酸中间体生产δ-癸内酯。
Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)-octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells.
作者信息
Kang Woo-Ri, Seo Min-Ju, An Jung-Ung, Shin Kyung-Chul, Oh Deok-Kun
机构信息
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
出版信息
Biotechnol Lett. 2016 May;38(5):817-23. doi: 10.1007/s10529-016-2041-3. Epub 2016 Jan 12.
OBJECTIVE
To produce δ-decalactone from linoleic acid by one-pot reaction using linoleate 13-hydratase with supplementation with whole Yarrowia lipolytica cells.
RESULTS
Whole Y. lipolytica cells at 25 g l(-1) produced1.9 g l(-1) δ-decalactone from 7.5 g 13-hydroxy-9(Z)-octadecenoic acid l(-1) at pH 7.5 and 30 °C for 21 h. Linoleate 13-hydratase from Lactobacillus acidophilus at 3.5 g l(-1) with supplementation with 25 g Y. lipolytica cells l(-1) in one pot at 3 h produced 1.9 g l(-1) δ-decalactone from 10 g linoleic acid l(-1) via 13-hydroxy-9(Z)-octadecenoic acid intermediate at pH 7.5 and 30°C after 18 h, with a molar conversion yield of 31 % and productivity of 106 mg l(-1) h(-1).
CONCLUSION
To the best of our knowledge, this is the first production of δ-decalactone using unsaturated fatty acid.
目的
通过一锅法反应，利用亚油酸13 - 水合酶并添加解脂耶氏酵母全细胞，从亚油酸生产δ-癸内酯。
结果
在pH 7.5和30℃条件下，25 g l⁻¹的解脂耶氏酵母全细胞在21小时内，从7.5 g l⁻¹的13 - 羟基-9(Z)-十八碳烯酸生产出1.9 g l⁻¹的δ-癸内酯。在3小时内，于pH 7.5和30℃条件下，将3.5 g l⁻¹嗜酸乳杆菌的亚油酸13 - 水合酶与25 g l⁻¹解脂耶氏酵母细胞在一锅法中添加，18小时后通过13 - 羟基-9(Z)-十八碳烯酸中间体从10 g l⁻¹亚油酸生产出1.9 g l⁻¹的δ-癸内酯，摩尔转化率为31%，生产率为106 mg l⁻¹ h⁻¹。
结论
据我们所知，这是首次使用不饱和脂肪酸生产δ-癸内酯。
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