新种，从蜱中分离得到。

sp. nov., isolated from the tick .

作者信息

Cabezas-Cruz Alejandro, Zweygarth Erich, Vancová Marie, Broniszewska Marzena, Grubhoffer Libor, Passos Lygia Maria Friche, Ribeiro Múcio Flávio Barbosa, Alberdi Pilar, de la Fuente José

机构信息

Center for Infection and Immunity of Lille (CIIL), INSERM U1019 - CNRS UMR 8204, Université de Lille, Institut Pasteur de Lille, 1 rue Professeur Calmette, 59019 Lille Cedex, France.

Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstrasse 5, 80802 Munich, Germany.

出版信息

Int J Syst Evol Microbiol. 2016 Mar;66(3):1426-1430. doi: 10.1099/ijsem.0.000895. Epub 2016 Jan 12.

DOI:10.1099/ijsem.0.000895

PMID:26763978

Abstract

Recently, we obtained a rickettsial isolate ( sp. UFMG-EV) from the haemolymph of engorged tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, , , and sequences we showed that sp. UFMG-EV belongs to the , family , genus . sp. UFMG-EV is a sister taxon of with 16S rRNA gene, , , and sequence similarities of 98.3 %, 97.2 %, 94.7 %, 94.3 % and 49.1 %, respectively. sp. UFMG-EV has been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and was found to resemble that of , and , but not and . We propose the name sp. nov. for this bacterium to acknowledge the place from where it was initially isolated, Minas Gerais, Brazil. The type strain is strain sp. UFMG-EV ( = DSM 100393 = TCB-TBB-0018).

摘要

最近，我们从饱血雌蜱的血淋巴中获得了一种立克次氏体分离株（UFMG-EV 种）。基于使用 16S rRNA 基因、、和序列的最大似然系统发育分析，我们表明 UFMG-EV 种属于科、属。UFMG-EV 种是的姐妹分类单元，其 16S rRNA 基因、、和序列相似性分别为 98.3%、97.2%、94.7%、94.3% 和 49.1%。UFMG-EV 种已通过在 IDE8 蜱细胞系中连续传代在实验室中保存，在那里使用电子显微镜对其超微结构进行了表征，发现其类似于、和的超微结构，但不同于和的。我们提议将这种细菌命名为新种，以认可其最初分离的地点，即巴西米纳斯吉拉斯州。模式菌株是 UFMG-EV 种菌株（= DSM 100393 = TCB-TBB-0018）。