Dusse Luci M, Godoi Lara C, Gomes Karina B, Carvalho Maria das G, Lwaleed Bashir A
aClinical and Toxicological Department, Faculty of Pharmacy, Federal University of Minas Gerais, BrazilbFaculty of Health Sciences, University of Southampton, Southampton, UK.
Blood Coagul Fibrinolysis. 2016 Jun;27(4):436-40. doi: 10.1097/MBC.0000000000000507.
Previously we investigated the tissue factor (TF)-dependent coagulation pathway and key haemostatic cofactors in white women with preeclampsia (P-EC) and suggested that plasma factor VII (FVII) levels can differentiate women with P-EC from healthy nonpregnant women or normal pregnant women, at the same trimester, with high sensitivity, specificity, positive and negative predictive values. Here we re-examine the TF-dependent pathway in a large cohort of Brazilian women. A total of 240 women were studied. These included healthy nonpregnant women (n = 79), normotensive pregnant women (n = 80) and women with severe P-EC (n = 81). Commercially available enzyme-linked immunosorbent assays were used to measure plasma FVII, activated factor VII (FVIIa), TF and tissue factor pathway inhibitor (TFPI). All study participants were matched for age. Pregnant women (with/without P-EC) were matched for gestational age and parity. Plasma levels of FVII, FVIIa and TFPI were significantly increased in women with severe P-EC compared with healthy nonpregnant women (P < 0.01) or normotensive pregnant women (P < 0.01). FVIIa was also higher in normotensive pregnant women compared with nonpregnant women (P < 0.01). However, no such significant trends were observed for plasma TF levels (P = 0.074). In conclusion, circulating FVII, FVIIa and TFPI were significantly elevated in women with severe P-EC in the absence of comparable changes in plasma TF levels. The present work is in agreement with our previous report on FVII levels in white women with P-EC. Thus, this lends further support to the notion that plasma FVII levels are potentially valuable diagnostic marker for P-EC, irrespective of ethnicity.
此前，我们研究了子痫前期白人女性（P-EC）中组织因子（TF）依赖性凝血途径及关键止血辅助因子，并提出血浆因子VII（FVII）水平可在同一孕期以高灵敏度、特异性、阳性和阴性预测值，将P-EC女性与健康非孕女性或正常孕妇区分开来。在此，我们在一大群巴西女性中重新审视TF依赖性途径。共研究了240名女性。其中包括健康非孕女性（n = 79）、血压正常的孕妇（n = 80）和重度P-EC女性（n = 81）。使用市售酶联免疫吸附测定法测量血浆FVII、活化因子VII（FVIIa）、TF和组织因子途径抑制剂（TFPI）。所有研究参与者年龄匹配。孕妇（有/无子痫前期）孕周和产次匹配。与健康非孕女性（P < 0.01）或血压正常的孕妇（P < 0.01）相比，重度P-EC女性的血浆FVII、FVIIa和TFPI水平显著升高。与非孕女性相比，血压正常的孕妇FVIIa也更高（P < 0.01）。然而，血浆TF水平未观察到此类显著趋势（P = 0.074）。总之，在血浆TF水平无可比变化的情况下，重度P-EC女性的循环FVII、FVIIa和TFPI显著升高。本研究结果与我们之前关于P-EC白人女性FVII水平的报告一致。因此，这进一步支持了以下观点：无论种族如何，血浆FVII水平都是子痫前期潜在有价值的诊断标志物。
