Arafa Reda Sanad, Abdel Haie Omima M, El-Azab Dina Shehata, Abdel-Rahman Amira Mohamed, Sira Mostafa M
Department of Pediatrics, Faculty of Medicine, Benha University, Egypt.
Department of Pathology, National Liver Institute, Menofiya University, 32511 Shebin El-koom, Menofiya, Egypt.
Cytokine. 2016 Mar;79:59-65. doi: 10.1016/j.cyto.2015.12.023. Epub 2016 Jan 5.
Although the exact etiology of biliary atresia (BA) is still elusive, inflammation plays a key role. Release of proinflammatory cytokines from activated immune cells perpetuates the injury and causes biliary destruction. We aimed to study interleukin (IL)-2 and IL-8 expression in liver tissue of BA patients compared with other neonatal cholestatic disorders.
The study included 59 infants with neonatal cholestasis in two groups; BA group (n=31) and non-BA group (n=28) with cholestatic disorders other than BA as controls. Demographic, clinical, laboratory, and histopathological parameters were collected. IL-2 and IL-8 immunostaining was performed. Immunostaining in portal cellular infiltrate was scored as positive or negative and expressed as the mean cell count in three portal tracts.
The mean value of IL-2 and IL-8 positive inflammatory cells was significantly higher in BA than in non-BA group (P-values of 0.004 and 0.002 respectively). IL-2 correlated significantly with IL-8 immunostaining in both BA and non-BA group (P<0.0001 for both). Furthermore, both cytokines in both groups correlated significantly with inflammatory activity in liver biopsy while there was no significant correlation with the other studied parameters. Yet, there was a trend of increased expression of IL-2 and IL-8 with increasing stage of fibrosis in BA group. This trend was not observed in non-BA group.
The significantly higher expression of IL-2 and IL-8 in patients with BA compared to non-BA suggests a potential role for these cytokines in the pathogenesis in therapy of this devastating neonatal hepatic disorder.
尽管胆道闭锁（BA）的确切病因仍不清楚，但炎症起关键作用。活化免疫细胞释放促炎细胞因子使损伤持续存在并导致胆管破坏。我们旨在研究与其他新生儿胆汁淤积性疾病相比，BA患者肝组织中白细胞介素（IL）-2和IL-8的表达情况。
本研究纳入两组共59例新生儿胆汁淤积患儿；BA组（n=31）和非BA组（n=28），后者为除BA外的其他胆汁淤积性疾病作为对照。收集人口统计学、临床、实验室及组织病理学参数。进行IL-2和IL-8免疫染色。对门管区细胞浸润的免疫染色进行阳性或阴性评分，并表示为三个门管区的平均细胞计数。
BA组IL-2和IL-8阳性炎症细胞的平均值显著高于非BA组（P值分别为0.004和0.002）。在BA组和非BA组中，IL-2与IL-8免疫染色均显著相关（两者P<0.0001）。此外，两组中的这两种细胞因子均与肝活检中的炎症活动显著相关，而与其他研究参数无显著相关性。然而，BA组中IL-2和IL-8的表达有随纤维化程度增加而升高的趋势。非BA组未观察到这种趋势。
与非BA患者相比，BA患者中IL-2和IL-8的表达显著更高，提示这些细胞因子在这种严重的新生儿肝脏疾病的发病机制及治疗中可能起作用。
