Chávez-Calvillo Gabriela, Contreras-Paredes Carlos A, Mora-Macias Javier, Noa-Carrazana Juan C, Serrano-Rubio Angélica A, Dinkova Tzvetanka D, Carrillo-Tripp Mauricio, Silva-Rosales Laura
Departamento de Ingeniería Genética, Unidad Irapuato.
Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados, Irapuato Guanajuato, Mexico.
Virology. 2016 Feb;489:179-91. doi: 10.1016/j.virol.2015.11.026. Epub 2016 Jan 5.
Antagonism between unrelated plant viruses has not been thoroughly described. Our studies show that two unrelated viruses, papaya ringspot virus (PRSV) and papaya mosaic virus (PapMV) produce different symptomatic outcomes during mixed infection depending on the inoculation order. Synergism occurs in plants infected first with PRSV or in plants infected simultaneously with PRSV and PapMV, and antagonism occurs in plants infected first with PapMV and later inoculated with PRSV. During antagonism, elevated pathogenesis-related (PR-1) gene expression and increased reactive oxygen species production indicated the establishment of a host defense resulting in the reduction in PRSV titers. Polyribosomal fractioning showed that PRSV affects translation of cellular eEF1α, PR-1, β-tubulin, and PapMV RNAs in planta, suggesting that its infection could be related to an imbalance in the translation machinery. Our data suggest that primary PapMV infection activates a defense response against PRSV and establishes a protective relationship with the papaya host.
不相关植物病毒之间的拮抗作用尚未得到充分描述。我们的研究表明，两种不相关的病毒，番木瓜环斑病毒（PRSV）和番木瓜花叶病毒（PapMV），在混合感染期间根据接种顺序会产生不同的症状结果。协同作用发生在首先感染PRSV的植物中或同时感染PRSV和PapMV的植物中，而拮抗作用发生在首先感染PapMV且随后接种PRSV的植物中。在拮抗作用期间，病程相关（PR-1）基因表达升高和活性氧产生增加表明建立了宿主防御，导致PRSV滴度降低。多核糖体分级分离表明，PRSV影响植物中细胞eEF1α、PR-1、β-微管蛋白和PapMV RNA的翻译，这表明其感染可能与翻译机制的失衡有关。我们的数据表明，原发性PapMV感染激活了针对PRSV的防御反应，并与番木瓜宿主建立了保护关系。
