Han Guang-jie, Li Chuan-ming, Sun Jun, Liu Qin, Zhao Song, Qi Jian-hang, Xu Jian
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015 Aug;27(4):385-9.
To investigate the effect of Bacillus thuringiensis var. israelensis (Bti) on the activities of three detoxification enzymes of Culex pipiens pallens and Aedes aegypti larvae.
The activities of glutathione transferase, acetyl cholinesterase and carboxyl esterase, were detected after two kinds of mosquito larvae were treated by Bti at different time and concentrations.
The activities of three detoxification enzymes of the two kinds of mosquito larvae were influenced by Bti treatment. The activity of glutathione transferase was increased after the Bti treatment, but declined significantly and inhibited at a low level sustainably. The activity of carboxyl esterase was increased after the Bti treatment, but recovered to normal level quickly. Acetyl cholinesterase was affected slightly by the Bti treatment, shortly inhibited in the first time and then recovered. The active effects of the three detoxification enzymes were positively related to the concentration of Bti.
The enzyme activities of glutathione transferase, acetyl cholinesterase and carboxyl esterase could be affected by Bti significantly.
研究苏云金芽孢杆菌以色列变种（Bti）对淡色库蚊和埃及伊蚊幼虫三种解毒酶活性的影响。
用不同时间和浓度的Bti处理两种蚊虫幼虫后，检测谷胱甘肽转移酶、乙酰胆碱酯酶和羧酸酯酶的活性。
Bti处理影响两种蚊虫幼虫三种解毒酶的活性。Bti处理后谷胱甘肽转移酶活性升高，但随后显著下降并持续处于低水平抑制状态。Bti处理后羧酸酯酶活性升高，但很快恢复到正常水平。乙酰胆碱酯酶受Bti处理影响较小，首次短暂抑制后恢复。三种解毒酶的活性效应与Bti浓度呈正相关。
Bti可显著影响谷胱甘肽转移酶、乙酰胆碱酯酶和羧酸酯酶的酶活性。
