Tan Mu-xiu, Zeng Wen-wen, Wei Peng-xiao, Mo Qiao-cheng, Pu Zu-ning, Cen Xiu-fen, Shi Feng-hua
Zhong Yao Cai. 2015 May;38(5):899-903.
To explore the germination conditions of Lonicera hypoglauca sand culture seeds and the effects of sand culture seedlings sterilization.
0.1% HgCl2 with different sterilization time, different illumination time and temperature culture condition were adopted to study the germination conditions of sand culture seeds. Different sterilization treatments and different hardening-seedling days were used to test the sterilization effect of sand culture seedlings.
The sterilization effect of the combination of 75% ethanol 30 s + 0.1% HgCl2 5 min on Lonicera hypoglauca seeds was the optimum,with the average pollution rate of 15.56%, and the average germination rate reached 51.11%. The combination of varied temperature-room temperature under light for 12 h/d was the best, with the average germination rate peaked at 75.49%, and the average germination potential reached 68.36%. The treatment of detergent liquor scrub-tap water wash on the part above the hypocotyl, which was sand cultured under the opening condition and had no root, showed the best sterilization effect, with the average pollution rate was zero, and the average survival rate peaked at 100.00%. The sterilization effect of sand culture seedlings, which was disinfected after cleaning by detergent liquor scrub-tap water wash after hardening-seeding for 30 days, was the best, with the average pollution rate of 50.00%, and the average survival rate of 100.00%.
The best sterilization effect is the combination of 75% ethanol 30 s + 0.1% HgCl2 5 min; Lighting for 12 h/d of varied temperature-room temperature is regarded as the optimum culture condition. The treatment of detergent liquor scrub-tap water wash treatment on the part above the hypocotyl,which is sand cultured under the opening condition and had no root, shows the best sterilization effect. For the sand culture seedlings, before inoculated in subculture medium, should be hardening-seedling for some days and sterilized after detergent liquor scrub-tap water wash.
探讨山银花沙培种子的萌发条件及沙培幼苗的消毒效果。
采用不同灭菌时间的0.1% HgCl₂、不同光照时间和温度培养条件研究沙培种子的萌发条件。采用不同的灭菌处理和不同的炼苗天数来测试沙培幼苗的消毒效果。
75%乙醇30 s + 0.1% HgCl₂ 5 min组合对山银花种子的消毒效果最佳，平均污染率为15.56%，平均发芽率达51.11%。变温-室温12 h/d光照组合最佳，平均发芽率最高达75.49%，平均发芽势达68.36%。对开口无生根沙培条件下的下胚轴以上部分采用洗洁精擦洗-自来水冲洗处理，消毒效果最佳，平均污染率为零，平均成活率最高达100.00%。炼苗30天后经洗洁精擦洗-自来水冲洗后消毒的沙培幼苗消毒效果最佳，平均污染率为50.00%，平均成活率为100.00%。
最佳消毒效果为75%乙醇30 s + 0.1% HgCl₂ 5 min组合；变温-室温12 h/d光照视为最佳培养条件。对开口无生根沙培条件下的下胚轴以上部分采用洗洁精擦洗-自来水冲洗处理，消毒效果最佳。对于沙培幼苗，转接至继代培养基前应进行一定天数的炼苗，并经洗洁精擦洗-自来水冲洗后消毒。
