Nashida T, Yoshimura K, Yoshie S, Mizuhashi F, Shimomura-Kuroki J
Departments of Biochemistry, The Nippon Dental University School of Life Dentistry at Niigata, Chuo-ku, Niigata, Japan.
Departments of Anatomy, The Nippon Dental University School of Life Dentistry at Niigata, Chuo-ku, Niigata, Japan.
Oral Dis. 2016 Jan;22(1):46-52. doi: 10.1111/odi.12377. Epub 2015 Nov 23.
To define the increased mRNA expression of Bpifb1, a member of the bactericidal/permeability-increasing protein family, in parotid acinar cells from non-obese diabetic (NOD) mice, an animal model for Sjögren's syndrome.
Parotid acinar cells were prepared from female NOD (NOD/ShiJcl) mice with or without diabetes, as well as from control (C57BL/6JJcl) mice. Total RNA and homogenate were prepared from the parotid acinar cells. Embryonic cDNA from a Mouse MTC(™) Panel I kit was used. The expression of Bpifb1 was determined by cDNA microarray analysis, RT-PCR, real-time PCR, northern blotting and in situ hybridization.
The expression of Bpifb1 mRNA was high in parotid acinar cells from diabetic and non-diabetic NOD mice at 5-50 weeks of age. Acinar cells in the C57BL/6 mice had a low expression of Bpifb1 mRNA at an age >8 weeks, but had a relatively high expression in the foetus and infantile stages.
Bpifb1 mRNA is upregulated in parotid acinar cells in NOD mice, but its expression is not related to the onset of diabetes. These findings suggest that high expression levels of Bpifb1 might predict disease traits before the onset of autoimmunity.
确定杀菌/通透性增加蛋白家族成员Bpifb1在非肥胖糖尿病（NOD）小鼠腮腺腺泡细胞中的mRNA表达增加，NOD小鼠是干燥综合征的动物模型。
从患有或未患糖尿病的雌性NOD（NOD/ShiJcl）小鼠以及对照（C57BL/6JJcl）小鼠中制备腮腺腺泡细胞。从腮腺腺泡细胞中制备总RNA和匀浆。使用来自Mouse MTC(™) Panel I试剂盒的胚胎cDNA。通过cDNA微阵列分析、逆转录-聚合酶链反应（RT-PCR）、实时定量聚合酶链反应（real-time PCR）、Northern印迹和原位杂交来测定Bpifb1的表达。
5至50周龄的糖尿病和非糖尿病NOD小鼠腮腺腺泡细胞中Bpifb1 mRNA的表达较高。C57BL/6小鼠的腺泡细胞在8周龄以上时Bpifb1 mRNA表达较低，但在胎儿和婴儿期表达相对较高。
NOD小鼠腮腺腺泡细胞中Bpifb1 mRNA上调，但其表达与糖尿病的发病无关。这些发现表明，Bpifb1的高表达水平可能在自身免疫发病前预测疾病特征。
