Nashida Tomoko, Shimomura-Kuroki Junko, Mizuhashi Fumi, Haga-Tsujimura Maiko, Yoshimura Ken, Hayashi-Sakai Sachiko
Department of Biochemistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
Department of Pediatric Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
Odontology. 2018 Apr;106(2):117-124. doi: 10.1007/s10266-017-0312-7. Epub 2017 Jul 26.
We previously showed that mRNA expression of BPIFB1 (Bpifb1), an antibacterial protein in the palate, lung, and nasal epithelium clone protein family, was increased in parotid acinar cells in non-obese diabetic (NOD, NOD/ShiJcl) mice, which is an animal model for Sjögren's syndrome. However, we did not previously assess the protein levels. In this report, we confirmed the expression of BPIFB1 protein in the parotid glands of NOD mice. Immunoblotting of subcellular fractions revealed that BPIBB1 was localised in secretory granules in parotid glands from NOD mice, and was almost not in parotid glands from the control mice. BPIFB1 had N-linked glycan that reacted with Aleuria aurantia lectin, which caused two types of spots with a slightly different pI and molecular weight. The expression of BPIFB1 protein was also demonstrated by immunohistochemistry. BPIFB1 was detected in the saliva from NOD mice but not in the saliva from the control mice, indicating individual constitution. BPIFB1 in saliva may be applied to other research as a diagnostic marker.
我们之前发现，腭、肺和鼻上皮克隆蛋白家族中的一种抗菌蛋白BPIFB1（Bpifb1）在非肥胖糖尿病（NOD，NOD/ShiJcl）小鼠的腮腺腺泡细胞中mRNA表达增加，NOD小鼠是干燥综合征的动物模型。然而，我们之前并未评估其蛋白水平。在本报告中，我们证实了BPIFB1蛋白在NOD小鼠腮腺中的表达。亚细胞组分的免疫印迹显示，BPIBB1定位于NOD小鼠腮腺的分泌颗粒中，而对照小鼠的腮腺中几乎没有。BPIFB1具有与橙黄网柄菌凝集素反应的N-连接聚糖，这导致了两种等电点和分子量略有不同的条带。免疫组织化学也证实了BPIFB1蛋白的表达。在NOD小鼠的唾液中检测到了BPIFB1，而对照小鼠的唾液中未检测到，这表明存在个体差异。唾液中的BPIFB1可能作为一种诊断标志物应用于其他研究。
