Deja M A, Malinowski M, Golba K S, Piekarska M, Wos S
Department of Cardiac Surgery, Medical University of Silesia, School of Medicine in Katowice, Poland.
Department of Electrocardiology and Heart Failure, Medical University of Silesia, School of Health Sciences in Katowice, Poland.
J Physiol Pharmacol. 2015 Dec;66(6):841-6.
Perivascular tissue (PVT) modulates vascular tone, releasing adventitia/adipocyte derived relaxing factor (ADRF). Its physiological role remains unclear. We studied isolated internal thoracic artery (ITA) segments obtained from 132 patients subjected to coronary artery bypass grafting. The vessels were skeletonized in vitro and the ITA rings and PVT were incubated in separate isolated organ baths. Skeletonized ITA segments were first precontracted with 10(-5.5)mol/L 5-hydroxytryptamine hydrochloride. The PVT was next transferred to the ITA tissue bath. This resulted in relaxation of ITA, presumably related to ADRF release from PVT which was floating freely in the tissue bath. The in-vitro relaxation responses were then correlated to patients' characteristics - including demographics, clinical and laboratory data, as well as therapy. Perivascular tissue transfer resulted in 49.7 ± 26.2% relaxation of precontracted ITA segments. In multiple linear regression modelling, the relaxation of ITAto PVT was negatively related to patient age (β = -0.67; 95% CI -1.17 - -0.17; P = 0.009), symptoms of CCS class 4 angina (β = -20.11; 95%CI -32.25 - -7.97; P = 0.001), and positively to body mass (β = 0.37; 95%CI 0.08 - 0.67; P = 0.01) and lack of heart failure symptoms (NYHA class 1) (β = 9.06; 95%CI 0.33 - 17.79; P = 0.04). The relaxation response to PVT was not related to patients' sex, diabetes, hypertension, lipid profile or therapy in both univariate and multivariate analysis. PVT might play an important role in regulating vascular tone in humans as exemplified by its changing physiological function with age and in atherosclerosis.
血管周围组织（PVT）通过释放外膜/脂肪细胞衍生的舒张因子（ADRF）来调节血管张力。其生理作用尚不清楚。我们研究了从132例行冠状动脉旁路移植术患者获取的离体胸廓内动脉（ITA）节段。血管在体外进行骨骼化处理，将ITA环和PVT分别置于独立的离体器官浴中孵育。首先用10^（-5.5）mol/L盐酸5-羟色胺使骨骼化的ITA节段预收缩。接下来将PVT转移至ITA组织浴中。这导致ITA舒张，推测与PVT释放ADRF有关，此时PVT在组织浴中自由漂浮。然后将体外舒张反应与患者特征相关联，包括人口统计学、临床和实验室数据以及治疗情况。血管周围组织转移导致预收缩的ITA节段舒张49.7±26.2%。在多元线性回归模型中，ITA对PVT的舒张反应与患者年龄呈负相关（β = -0.67；95%可信区间-1.17至-0.17；P = 0.009），与加拿大心血管学会（CCS）4级心绞痛症状呈负相关（β = -20.11；95%可信区间-32.25至-7.97；P = 0.001），与体重呈正相关（β = 0.37；95%可信区间0.08至0.67；P = 0.01），与无心力衰竭症状（纽约心脏协会[NYHA]1级）呈正相关（β = 9.06；95%可信区间0.33至17.79；P = 0.04）。在单因素和多因素分析中，对PVT的舒张反应与患者性别、糖尿病、高血压、血脂谱或治疗均无关。PVT可能在调节人体血管张力中发挥重要作用，这在其随年龄变化的生理功能以及动脉粥样硬化中得到体现。
