Wang Lujia, Feng Chenchen, Ding Guanxiong, Jiang Haowen, Ding Qiang, Wu Zhong
Department of Urology, Huashan Hospital, Fudan University Shanghai 200040, PR China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18819-24. eCollection 2015.
To study the potential of BLCA1 for detection of urothelial cancer including urinary bladder cancer (UBC) and upper tract urothelial cancer (UTUC).
An antibody for BLCA1 was generated and indirect ELISA was used to detect urinary BLCA1 level. Clinicopathological parameters were studied for the association with BLCA1 level. Urine samples from UBC and UTUC patients, together with cases with other urological disorders were collected and tested.
Urinary BLCA1 level in UBC and UTUC patients were significantly higher than that in normal controls. This was also proven when urothelial cancers were grouped as a whole, where as the level did not differ between UBC and UTUC samples. BLCA1 was also significantly higher in urothelial cancer samples compared to other urological disorders such as patients with long-dwelling catheter, glandular cystitis, BPH and other benign conditions. Cut-off value at 0.0009 OD/μg yielded a sensitivity of 92.7% and a specificity of 92.9% for UBC.
Urinary BLCA1 is a promising marker for urothelial cancers including UBC and UTUC with high sensitivity and specificity.
研究BLCA1在检测尿路上皮癌（包括膀胱癌（UBC）和上尿路尿路上皮癌（UTUC））中的潜力。
制备针对BLCA1的抗体，并使用间接酶联免疫吸附测定法检测尿液中BLCA1水平。研究临床病理参数与BLCA1水平的相关性。收集UBC和UTUC患者以及患有其他泌尿系统疾病患者的尿液样本并进行检测。
UBC和UTUC患者尿液中的BLCA1水平显著高于正常对照组。当将尿路上皮癌作为一个整体进行分组时也得到了证实，不过UBC和UTUC样本中的水平并无差异。与其他泌尿系统疾病（如长期留置导尿管患者、腺性膀胱炎、良性前列腺增生症及其他良性疾病）相比，尿路上皮癌样本中的BLCA1水平也显著更高。对于UBC，0.0009 OD/μg的临界值产生了92.7%的灵敏度和92.9%的特异性。
尿液中的BLCA1是一种有前景的尿路上皮癌标志物，对UBC和UTUC具有高灵敏度和特异性。
