Jiang Xing-Wang, Li Xiao-Rui, Zhang Yan-Ping
Department of Otolaryngology, 309th Hospital of Chinese PLA Beijing 100091, China.
Department of Otolaryngology, Yuncheng Central Hospital Yuncheng 044099, Shanxi, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):19058-64. eCollection 2015.
To investigate the changes of ribbon synapses (RS) number in cochlear hair cells in C57BL/6J mice with age. Basilar membranes within the cochlea of C57BL/6J mice aged 2, 6, 10 and 12 months were harvested (5 mice in each age group). The presynaptic and postsynaptic membranes were subject to double immunohistochemical staining and observed with a laser confocal microscope. The number of RS in each segment of basilar membrane was counted by using 3D reconstruction technique. Compared with 2-month-old mice, reduction of RS number in basilar membrane inside cochlea mainly occurred to the basal turn among C57BL/6J mice aged 6 months. The number of RS in each turn among 10-month-old mice decreased considerably, and such decrease continued in the top turn and middle turn in mice aged 12 months. In contrast, the number of RS in the basal turn increased slightly. Reduction of RS probably takes place in the early stage of C57BL/6J mice presbycusis. Early prevention of presbycusis can be achieved through measures to mitigate the reduction of RS.
研究C57BL/6J小鼠耳蜗毛细胞中带状突触（RS）数量随年龄的变化。采集2、6、10和12月龄C57BL/6J小鼠耳蜗内的基底膜（每个年龄组5只小鼠）。对突触前膜和突触后膜进行双重免疫组织化学染色，并用激光共聚焦显微镜观察。采用三维重建技术计数基底膜各段的RS数量。与2月龄小鼠相比，6月龄C57BL/6J小鼠耳蜗内基底膜RS数量减少主要发生在蜗底。10月龄小鼠各圈RS数量均显著减少，12月龄小鼠蜗顶和蜗中RS数量持续减少，蜗底RS数量略有增加。RS数量减少可能发生在C57BL/6J小鼠老年性聋的早期阶段。通过采取措施减轻RS数量减少可实现老年性聋的早期预防。
