Kim Junhwan, Villarroel José Paul Perales, Zhang Wei, Yin Tai, Shinozaki Koichiro, Hong Angela, Lampe Joshua W, Becker Lance B
Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
College of Liberal Arts and Sciences, Villanova University, Villanova, PA 19085, USA.
Oxid Med Cell Longev. 2016;2016:7463407. doi: 10.1155/2016/7463407. Epub 2015 Dec 7.
Cardiac arrest induces whole-body ischemia, which causes damage to multiple organs. Understanding how each organ responds to ischemia/reperfusion is important to develop better resuscitation strategies. Because direct measurement of organ function is not practicable in most animal models, we attempt to use mitochondrial respiration to test efficacy of resuscitation on the brain, heart, kidney, and liver following prolonged cardiac arrest. Male Sprague-Dawley rats are subjected to asphyxia-induced cardiac arrest for 30 min or 45 min, or 30 min cardiac arrest followed by 60 min cardiopulmonary bypass resuscitation. Mitochondria are isolated from brain, heart, kidney, and liver tissues and examined for respiration activity. Following cardiac arrest, a time-dependent decrease in state-3 respiration is observed in mitochondria from all four tissues. Following 60 min resuscitation, the respiration activity of brain mitochondria varies greatly in different animals. The activity after resuscitation remains the same in heart mitochondria and significantly increases in kidney and liver mitochondria. The result shows that inhibition of state-3 respiration is a good marker to evaluate the efficacy of resuscitation for each organ. The resulting state-3 respiration of brain and heart mitochondria following resuscitation reenforces the need for developing better strategies to resuscitate these critical organs following prolonged cardiac arrest.
心脏骤停会引发全身缺血，进而对多个器官造成损害。了解每个器官对缺血/再灌注的反应对于制定更好的复苏策略至关重要。由于在大多数动物模型中直接测量器官功能并不可行，我们尝试利用线粒体呼吸来测试长时间心脏骤停后对脑、心脏、肾脏和肝脏的复苏效果。将雄性Sprague-Dawley大鼠进行窒息诱导的心脏骤停30分钟或45分钟，或30分钟心脏骤停后进行60分钟体外循环复苏。从脑、心脏、肾脏和肝脏组织中分离出线粒体，并检测其呼吸活性。心脏骤停后，在所有四个组织的线粒体中均观察到状态3呼吸随时间下降。复苏60分钟后，脑线粒体的呼吸活性在不同动物中差异很大。复苏后心脏线粒体的活性保持不变，而肾脏和肝脏线粒体的活性显著增加。结果表明，抑制状态3呼吸是评估每个器官复苏效果的良好指标。复苏后脑和心脏线粒体的状态3呼吸结果进一步强调了在长时间心脏骤停后需要制定更好的策略来复苏这些关键器官。
