单核细胞血管紧张素与内皮素受体失衡调节促纤维化趋化因子配体18的分泌。
Monocytic Angiotensin and Endothelin Receptor Imbalance Modulate Secretion of the Profibrotic Chemokine Ligand 18.
作者信息
Rademacher Judith, Kill Angela, Mattat Kathrin, Dragun Duska, Siegert Elise, Günther Jeannine, Riemekasten Gabriela
机构信息
From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
出版信息
J Rheumatol. 2016 Mar;43(3):587-91. doi: 10.3899/jrheum.150474. Epub 2016 Jan 15.
OBJECTIVE
To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance.
METHODS
Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA.
RESULTS
Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion.
CONCLUSION
Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.
目的
评估系统性硬化症（SSc）中血管紧张素和内皮素受体的单核细胞表达及比例及其功能相关性。
方法
通过流式细胞术检测受体表达。采用酶联免疫吸附测定法（ELISA）测量用免疫球蛋白G刺激的外周血单核细胞上清液中趋化因子配体18（CCL18）的浓度。
结果
与健康供者相比，SSc患者的单核细胞呈现出血管紧张素II 1型受体（AT1R）/AT2R比例升高。肺纤维化患者和改良Rodnan皮肤评分高的患者显示内皮素1 A型受体（ETAR）/ETBR比例降低。高AT1R/AT2R但低ETAR/ETBR比例对应于更高的CCL18分泌。
结论
在SSc中观察到的血管紧张素和内皮素受体比例改变影响自身抗体介导的效应，如促纤维化CCL18的分泌。
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