使用Fe3O4/Cu-磷灰石纳米颗粒对组氨酸标签蛋白进行选择性结合和磁分离。
Selective binding and magnetic separation of histidine-tagged proteins using Fe3O4/Cu-apatite nanoparticles.
作者信息
Li Ping, Li Liangliang, Zhao Yanbao, Sun Lei, Zhang Yu
机构信息
Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, PR China.
Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
出版信息
J Inorg Biochem. 2016 Mar;156:49-54. doi: 10.1016/j.jinorgbio.2015.12.017. Epub 2015 Dec 30.
Hierarchical Fe3O4@Cu-apatite nanoparticles (NPs) were synthesized via a facile hydrothermal method. The Fe3O4 cores present spherical shape and have a mean diameter of 300nm, and the Cu-apatite shell with thickness of about 50nm is composed of a large number of sheets. Using the high affinity of Cu(2+) on the surface toward histidine tags, the Fe3O4@Cu-apatite NPs can be applied to enrich and magnetically separate histidine tagged (His-tagged) proteins directly from the mixture of lysed cells. Research results indicated that the Fe3O4@Cu-apatite NPs presented negligible nonspecific protein adsorption and high protein binding ability.
通过一种简便的水热法合成了分级结构的Fe3O4@Cu-磷灰石纳米颗粒（NPs）。Fe3O4核呈球形，平均直径为300nm，厚度约为50nm的Cu-磷灰石壳由大量薄片组成。利用表面Cu(2+)对组氨酸标签的高亲和力，Fe3O4@Cu-磷灰石NPs可直接用于从裂解细胞混合物中富集和磁性分离组氨酸标记（His-tagged）的蛋白质。研究结果表明，Fe3O4@Cu-磷灰石NPs的非特异性蛋白质吸附可忽略不计，且具有高蛋白结合能力。
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