Le Tao, Zhu Liqian, Yu Huan
College of Life Science, Chongqing Normal University, Chongqing 401331, PR China.
College of Veterinary, Yangzhou University, Yangzhou 225009, PR China.
Food Chem. 2016 May 15;199:70-4. doi: 10.1016/j.foodchem.2015.11.116. Epub 2015 Nov 26.
A novel and reliable dual-label direct competitive fluorescence-linked immunosorbent assay (dc-FLISA) based on quantum dots (QDs) was developed for the simultaneous determination of the major metabolites of Carbadox and Olaquindox residues in animal tissues, using anti-QCA monoclonal antibodies and anti-MQCA polyclonal antibodies labeled with QD520 and QD635, respectively. The limits of detection for QCA and MQCA were 0.05 and 0.07ng/ml, respectively. The method was used to analyze fortified samples and analyte recoveries ranged from 81.5% to 98.2% (QCA) and 84.2% to 95.7% (MQCA). Good correlations between the dc-FLISA method and HPLC were demonstrated for the determination of QCA and MQCA residues in swine tissue samples, confirming the reliability of the proposed method.
基于量子点（QDs）开发了一种新颖且可靠的双标记直接竞争荧光联免疫吸附测定法（dc-FLISA），用于同时测定动物组织中卡巴多司和喹乙醇残留的主要代谢物，分别使用标记有QD520和QD635的抗喹乙醇酸（QCA）单克隆抗体和抗3-甲基喹喔啉-2-羧酸（MQCA）多克隆抗体。QCA和MQCA的检测限分别为0.05和0.07ng/ml。该方法用于分析加标样品，分析物回收率在81.5%至98.2%（QCA）和84.2%至95.7%（MQCA）之间。在猪组织样品中QCA和MQCA残留量的测定方面，dc-FLISA方法与高效液相色谱法（HPLC）之间显示出良好的相关性，证实了所提出方法的可靠性。
