Egozi Dana, Shandalov Yulia, Freiman Alina, Rosenfeld Dekel, Ben-Shimol David, Levenberg Shulamit
Department of Plastic Surgery, Kaplan Medical Center.
Biomedical Engineering, Technion Israel Institute of Technology.
J Vis Exp. 2016 Jan 11(107):52984. doi: 10.3791/52984.
One of the main factors limiting the thickness of a tissue construct and its consequential viability and applicability in vivo, is the control of oxygen supply to the cell microenvironment, as passive diffusion is limited to a very thin layer. Although various materials have been described to restore the integrity of full-thickness defects of the abdominal wall, no material has yet proved to be optimal, due to low graft vascularization, tissue rejection, infection, or inadequate mechanical properties. This protocol describes a means of engineering a fully vascularized flap, with a thickness relevant for muscle tissue reconstruction. Cell-embedded poly L-lactic acid/poly lactic-co-glycolic acid constructs are implanted around the mouse femoral artery and vein and maintained in vivo for a period of one or two weeks. The vascularized graft is then transferred as a flap towards a full thickness defect made in the abdomen. This technique replaces the need for autologous tissue sacrifications and may enable the use of in vitro engineered vascularized flaps in many surgical applications.
限制组织构建物厚度及其在体内相应的生存能力和适用性的主要因素之一，是对细胞微环境的氧气供应的控制，因为被动扩散仅限于非常薄的一层。尽管已经描述了各种材料来恢复腹壁全层缺损的完整性，但由于移植血管化程度低、组织排斥、感染或机械性能不足，尚未有材料被证明是最佳的。本方案描述了一种构建完全血管化皮瓣的方法，其厚度与肌肉组织重建相关。将细胞包埋的聚L-乳酸/聚乳酸-乙醇酸构建物植入小鼠股动脉和静脉周围，并在体内维持一到两周。然后将血管化移植物作为皮瓣转移到腹部制造的全层缺损处。这项技术取代了自体组织牺牲的需求，并可能使体外工程化血管化皮瓣在许多外科手术应用中得以使用。
