Xing Bao-Shan, Guo Qiong, Jiang Xiao-Yan, Chen Qian-Qian, Li Peng, Ni Wei-Min, Jin Ren-Cun
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou, 310036, China.
Appl Microbiol Biotechnol. 2016 May;100(10):4637-49. doi: 10.1007/s00253-016-7292-3. Epub 2016 Jan 16.
Preserving active anaerobic ammonium oxidation (anammox) biomass is a potential method for securing sufficient seeding biomass for the rapid start-up of full-scale anammox processes. In this study, anammox granules were cultured in an upflow anaerobic sludge blanket (UASB) reactor (R0), and then the enriched anammox granules were preserved at 35, 20, 4, and -30 °C. The subsequent reactivation characteristics of the granules were evaluated in four UASB reactors (denoted R1, R2, R3, and R4, respectively) to investigate the effect of preservation temperature on the characteristics of anammox granules and their reactivation performance. The results demonstrated that 4 °C was the optimal preservation temperature for maintaining the biomass, activity, settleability, and integrity of the anammox granules and their cellular structures. During the preservation period, a first-order exponential decay model may be used to simulate the decay of anammox biomass and activity. The protein-to-polysaccharide ratio in the extracellular polymeric substances and the heme c content could not effectively indicate the changes in settleability and activity of the anammox granules, respectively, and a loss of bioactivity was positively associated with the degree of anaerobic ammonium-oxidizing bacteria cell lysis. After 42 days of storage, the anammox granules preserved at 4 °C (R3) exhibited a better recovery performance than those preserved at 20 °C (R2), -30 °C (R4), and 35 °C (R1). The comprehensive comparison indicated that 4 °C is the optimal storage temperature for anammox granular sludge because it promotes improved maintenance and recovery performance properties.
保存活性厌氧氨氧化（anammox）生物质是确保有足够的接种生物质以实现全规模厌氧氨氧化工艺快速启动的一种潜在方法。在本研究中，厌氧氨氧化颗粒在升流式厌氧污泥床（UASB）反应器（R0）中培养，然后将富集的厌氧氨氧化颗粒分别保存在35、20、4和 -30℃ 下。在四个UASB反应器（分别记为R1、R2、R3和R4）中评估颗粒随后的再活化特性，以研究保存温度对厌氧氨氧化颗粒特性及其再活化性能的影响。结果表明，4℃ 是维持厌氧氨氧化颗粒及其细胞结构的生物质、活性、沉降性能和完整性的最佳保存温度。在保存期间，一级指数衰减模型可用于模拟厌氧氨氧化生物质和活性的衰减。胞外聚合物中的蛋白质与多糖比率和血红素c含量分别不能有效指示厌氧氨氧化颗粒沉降性能和活性的变化，生物活性的丧失与厌氧氨氧化细菌细胞裂解程度呈正相关。储存42天后，保存在4℃（R3）的厌氧氨氧化颗粒比保存在20℃（R2）、-30℃（R4）和35℃（R1）的颗粒表现出更好的恢复性能。综合比较表明，4℃ 是厌氧氨氧化颗粒污泥的最佳储存温度，因为它能促进更好的维持和恢复性能。
