Ramrakha P S, Sharp R J, Yeoman H, Stanley M A
Department of Pathology, University of Cambridge, England.
Transplantation. 1989 Oct;48(4):676-80.
Our group has shown previously that APC-depleted cultured epidermal keratinocytes show prolonged survival when grafted onto normal MHC-incompatible adult mice. We show here that in vitro culture also improves significantly the survival of MHC-compatible keratinocyte allografts, although these nonrejected grafts are repopulated by host cells identified by their dendritic morphology and phenotype (class II+, leukocyte-common antigen+) as APCs. Reconstitution of cultured grafts, immediately prior to transplantation, with MHC-compatible dendritic cells of either donor or recipient origin, results in graft rejection--thus demonstrating that cultured cells can be rejected by the recipient animal--and suggests that a paucity of APCs in the immediate postgrafting period is responsible for the privilege afforded these grafts.
我们的研究小组之前已经表明，将缺乏APC的培养表皮角质形成细胞移植到正常的MHC不相容成年小鼠身上时，其存活时间会延长。我们在此表明，体外培养也能显著提高MHC相容的角质形成细胞同种异体移植物的存活率，尽管这些未被排斥的移植物会被宿主细胞重新填充，这些宿主细胞通过其树突状形态和表型（II类阳性、白细胞共同抗原阳性）被鉴定为APC。在移植前立即用供体或受体来源的MHC相容树突状细胞重建培养的移植物，会导致移植物排斥——从而证明培养的细胞会被受体动物排斥——并表明移植后即刻APC的缺乏是这些移植物具有免疫特权的原因。
