Li Pei, Han Yitao, Li Yong, Zhu Rong, Wang Huixia, Nie Zhou, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, China.
Anal Bioanal Chem. 2016 Apr;408(11):2659-68. doi: 10.1007/s00216-016-9304-7. Epub 2016 Jan 21.
Reversible protein acetylation catalyzed by histone acetyltransferases (HATs) and histone deacetylases (HDACs) is an essential post-translational modification (PTM) mechanism which correlates largely with epigenetic gene regulation such as transcriptional activation, DNA replication, histone deposition, and DNA repair. Dysfunction of histone acetylation and the aberrant activity of HATs/HDACs is often associated with the pathogenesis of numerous diseases, especially cancer. Therefore, developing potent and specific analytical methods for HATs/HDACs is important for fundamental biochemical research, disease diagnosis and treatment, and drug development. This paper briefly summarizes the general design strategies used in HAT/HDAC sensors, gives a systematic overview of recent advances in the analytical methods for HAT/HDAC enzymatic analysis, classifies these methods by their biorecognition mechanisms and relative applications either in vitro or in living cells, then outlines challenges faced by these bioanalytical methods and offers perspectives on future developments.
由组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）催化的可逆蛋白质乙酰化是一种重要的翻译后修饰（PTM）机制，在很大程度上与表观遗传基因调控相关，如转录激活、DNA复制、组蛋白沉积和DNA修复。组蛋白乙酰化功能障碍以及HATs/HDACs的异常活性通常与多种疾病，尤其是癌症的发病机制有关。因此，开发针对HATs/HDACs的高效、特异性分析方法对于基础生化研究、疾病诊断与治疗以及药物开发具有重要意义。本文简要总结了HAT/HDAC传感器中使用的一般设计策略，系统概述了HAT/HDAC酶分析方法的最新进展，根据其生物识别机制以及在体外或活细胞中的相关应用对这些方法进行分类，然后概述了这些生物分析方法面临的挑战，并对未来发展提出了展望。
