Miranda Carla, Parrish Colin R, Thompson Gertrude
Department of Veterinary Clinics, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, 4050-313 Porto, Portugal; Rede de Investigação em Biodiversidade e Biologia Evolutiva (CIBIO/InBIO), Laboratório Associado, Universidade do Porto, 4485-661 Vairão, Portugal.
Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Vet Microbiol. 2016 Feb 1;183:37-42. doi: 10.1016/j.vetmic.2015.11.037. Epub 2015 Nov 30.
Since its emergence, canine parvovirus type 2 (CPV-2) has caused disease pandemics with severe gastroenteritis signs, infecting especially puppies. As a consequence of CPV rapid evolution a variety of genetic and antigenic variants have been reported circulating worldwide. The detection of additional variants of CPV circulating in the dog population in Portugal suggests monitoring of the disease is useful. The objectives of this study were to further detect and characterize circulating field variants from suspected CPV diseased dogs that were admitted to veterinary clinics distributed throughout the country, during 2012-2014. Of the 260 fecal samples collected, 198 were CPV positive by PCR, and CPV antigen was detected in 61/109 samples by Immunochromatographic (IC) test. The restriction fragment length polymorphism (RFLP) analysis of 167 samples revealed that 86 were the CPV-2c. Sequence analysis of the 198 strains confirmed that CPV-2c were the dominant variant (51.5%), followed by CPV-2b (47.5%) and CPV-2a (1%). The variants were irregularly distributed throughout the country and some were detected with additional non-synonymous mutations in the VP2 gene. Phylogenetic analysis demonstrated that the isolates were similar to other European strains, and that this virus continues to evolve.
自出现以来，犬细小病毒2型（CPV-2）引发了伴有严重肠胃炎症状的疾病大流行，尤其感染幼犬。由于CPV的快速进化，已报道全球范围内有多种基因和抗原变体在传播。在葡萄牙犬类群体中检测到CPV的其他变体，这表明对该疾病进行监测是有用的。本研究的目的是进一步检测和鉴定2012年至2014年期间全国各地兽医诊所收治的疑似CPV患病犬所携带的流行野外变体。在收集的260份粪便样本中，198份通过PCR检测为CPV阳性，61/109份样本通过免疫层析（IC）试验检测到CPV抗原。对167份样本的限制性片段长度多态性（RFLP）分析显示，86份为CPV-2c。对198个毒株的序列分析证实，CPV-2c是主要变体（51.5%），其次是CPV-2b（47.5%）和CPV-2a（1%）。这些变体在全国分布不均，并且在VP2基因中检测到一些伴有额外非同义突变的变体。系统发育分析表明，分离株与其他欧洲毒株相似，并且这种病毒仍在继续进化。
