Carr L G, Xu Y, Ho W H, Edenberg H J
Department of Biochemistry, Indiana University School of Medicine, Indianapolis 46223.
Alcohol Clin Exp Res. 1989 Aug;13(4):594-6. doi: 10.1111/j.1530-0277.1989.tb00383.x.
All nine exons of the ADH2(3) allele, which encodes the human alcohol dehydrogenase beta 3 subunit, have been cloned and sequenced. Comparison of this sequence to the ADH2(1) nucleotide sequence revealed only a single difference that results in an amino acid change, thus proving that the significant kinetic differences between these two isozymes is due to the Cys for Arg substitution at position 369. There are also two silent polymorphisms, and two changes in noncoding regions.
编码人类乙醇脱氢酶β3亚基的ADH2(3)等位基因的全部9个外显子已被克隆和测序。将该序列与ADH2(1)核苷酸序列进行比较，结果仅发现一个导致氨基酸改变的差异，从而证明这两种同工酶之间显著的动力学差异是由于369位的半胱氨酸被精氨酸取代所致。此外，还有两个沉默多态性以及非编码区的两处变化。
