依维莫司洗脱支架降低单核细胞中Toll样受体4的表达。

Everolimus-Eluting Stents Reduce Monocyte Expression of Toll-Like Receptor 4.

作者信息

Shokri Mehriar, Bagheri Bahador, Garjani Alireza, Sohrabi Bahram, Habibzadeh Afshin, Kazemi Babak, Movassaghpour Ali Akbar

机构信息

Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Cancer Research Center and Department of Pharmacology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.

出版信息

Adv Pharm Bull. 2015 Dec;5(Suppl 1):643-7. doi: 10.15171/apb.2015.087. Epub 2015 Dec 31.

DOI:10.15171/apb.2015.087

PMID:26793610

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4708035/

Abstract

PURPOSE

Toll-like receptors (TLR) are well known components of the innate immune system. Among them, TLR4 is related to the inflammatory processes involved in atherosclerotic plaque formation. Our purpose was to compare the monocytic expression of TLR4 following implantation of drug-eluting (DES) and bare stents (BMS).

METHODS

In this study, patients with chronic stable angina undergoing elective percutaneous coronary intervention (PCI) in ShahidMadani Heart Hospital, Tabriz, Iran were included. Ninety-five patients receiving DES and 95 patients receiving BMS were selected between 2012 and 2014.Everolimus eluting stents were implanted for DES group. Both groups received similar medications and procedure. Blood samples were taken before PCI, 2 hours and 4 hours after termination of PCI. Expression of TLR4 on monocytes was measured using flowcytometry. Patients were matched for age, sex and coronary artery disease risk factors, but not for TLR4 expression rate before PCI.

RESULTS

A significant difference was seen between DES and BMS in TLR4 expression before (21.3±2.8% vs. 15.5±2.7%; P< 0.05) and four hours after PCI (30.1 ± 3.3% vs 39.2 ± 3.2%, P< 0.05). Due to the unmatched rate of TLR4+ expression before PCI, we measured the percentage of increase in TLR4 expression between groups. DES compared to BMS significantlycaused less increase in the TLR4 expression (50.23%±10.03% vs. 446.35%±70.58%, p<0.001).

CONCLUSION

Our findings suggest thateverolimuseluted from the stents can decrease PCI induced increase in the TLR4 expression on the surface of monocytes.

摘要

目的

Toll样受体（TLR）是先天性免疫系统的知名组成部分。其中，TLR4与动脉粥样硬化斑块形成所涉及的炎症过程相关。我们的目的是比较药物洗脱支架（DES）和裸金属支架（BMS）植入后单核细胞中TLR4的表达情况。

方法

本研究纳入了在伊朗大不里士的沙希德·马达尼心脏医院接受择期经皮冠状动脉介入治疗（PCI）的慢性稳定型心绞痛患者。在2012年至2014年间，选择了95例接受DES的患者和95例接受BMS的患者。DES组植入依维莫司洗脱支架。两组接受相似的药物治疗和手术操作。在PCI前、PCI结束后2小时和4小时采集血样。使用流式细胞术测量单核细胞上TLR4的表达。患者在年龄、性别和冠状动脉疾病危险因素方面进行了匹配，但PCI前的TLR4表达率未进行匹配。

结果

DES组和BMS组在PCI前（21.3±2.8%对15.5±2.7%；P<0.05）和PCI后4小时（30.1±3.3%对39.2±3.2%，P<0.05）的TLR4表达存在显著差异。由于PCI前TLR4+表达率不匹配，我们测量了两组之间TLR4表达增加的百分比。与BMS相比，DES显著导致TLR4表达增加较少（50.23%±10.03%对446.35%±70.58%，p<0.001）。