[初治HIV/AIDS患者CD8(+)T细胞中HLA-DR或CD38表达与CD4(+)T细胞计数之间的相关性]

[The correlation between HLA-DR or CD38 expression in CD8(+)T cells and CD4(+)T cells count in treatment-naive HIV/AIDS patients].

作者信息

Xie Jing, Qiu Zhifeng, Han Yang, Li Yanling, Song Xiaojing, Li Taisheng

机构信息

Department of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2016 Jan;55(1):36-9. doi: 10.3760/cma.j.issn.0578-1426.2016.01.009.

DOI:10.3760/cma.j.issn.0578-1426.2016.01.009

PMID:26796651

Abstract

OBJECTIVE

To analyze the correlation between CD4(+)T cell count and HLA-DR or CD38 expression on peripheral CD8(+)T cells in treatment-naive Chinese HIV/AIDS patients.

METHODS

A total of 471 treatment-naive HIV-infected patients and 53 healthy volunteers were enrolled. HLA-DR or CD38 expression on peripheral CD8(+)T cells, CD4(+)T cell count, naive CD4(+)T cell subsets and CD28 expression on T cells were measured by flow cytometry. Plasma HIV RNA load was quantified by real-time PCR (COBAS TaqMan RT-PCR). Mann-Whitney U test and Spearman's rank correlation test were used for statistical analysis.

RESULTS

CD38 expression intensity on CD8(+)T cells was negatively correlated with CD4(+)T cell count (r = -0.53, P < 0.001) and naive CD4(+)T cell count (r = -0.48, P < 0.001). CD38 expression on CD8(+)T cells also displayed significantly negative correlation with CD28 expression on CD8(+)T cells (r = -0.46, P < 0.001). HLA-DR expression on CD8(+)T cells did not show significant correlation with CD4(+)T cell count. Only weak positive correlation was found between CD38 intensity on CD8(+)T cells and plasma HIV RNA load.

CONCLUSIONS

Compared with HLA-DR, CD38 expression on CD8(+)T cells shows more significant negative correlation with CD4(+)T cell count in treatment-naive patients. CD38 and HLA-DR may play different roles on the persistent immune activation in HIV infection.

摘要

目的

分析未经治疗的中国艾滋病病毒/艾滋病（HIV/AIDS）患者外周血CD8(+)T细胞上CD4(+)T细胞计数与HLA-DR或CD38表达之间的相关性。

方法

共纳入471例未经治疗的HIV感染患者和53名健康志愿者。采用流式细胞术检测外周血CD8(+)T细胞上HLA-DR或CD38表达、CD4(+)T细胞计数、初始CD4(+)T细胞亚群及T细胞上CD28表达。采用实时荧光定量PCR（COBAS TaqMan RT-PCR）定量检测血浆HIV RNA载量。采用Mann-Whitney U检验和Spearman等级相关检验进行统计学分析。

结果

CD8(+)T细胞上CD38表达强度与CD4(+)T细胞计数呈负相关（r = -0.53，P < 0.001），与初始CD4(+)T细胞计数呈负相关（r = -0.48，P < 0.001）。CD8(+)T细胞上CD38表达与CD8(+)T细胞上CD28表达也呈显著负相关（r = -0.46，P < 0.001）。CD8(+)T细胞上HLA-DR表达与CD4(+)T细胞计数无显著相关性。CD8(+)T细胞上CD38强度与血浆HIV RNA载量仅呈弱正相关。