Ramzaoui S, Jouen-Beades F, Gilbert D, Borsa-Lebas F, Michel Y, Humbert G, Tron F
Groupe de Recherche en Immunopathologie, Institut Fédératif de Recherche Multidisciplinaire sur les Peptides, France.
Clin Immunol Immunopathol. 1995 Oct;77(1):33-41. doi: 10.1016/0090-1229(95)90134-5.
Three-color automated flow cytometry was carried out on peripheral blood CD4+ and CD8+ T-lymphocytes of 42 HIV-positive patients using tri-color anti-CD4 or anti-CD8, phycoerythrin-anti-CD38, and fluorescein-anti-HLA-DR, mAbs to elucidate further the T-cell activation hypothesis recently proposed to explain CD4+ T-cell abnormalities observed during HIV infection. CD4+ CD38+ T-cells constituted the major part of circulating CD4+ T-cells in HIV-infected patients and their HLA-DR molecule positivity increased as their disease progressed. The level of CD38 and HLA-DR expression on CD4+ T-cells was positively correlated to that of CD8+ T-cells and to the level of beta 2-microglobulin. Next, to determine whether CD38 expression was associated with a selective expansion or deletion of V beta gene-defined subsets, we compared the V beta gene frequencies between CD38+ and CD38- T-cells from HIV-infected CDC stage II patients using 13 mAbs specific to V beta families. While selective expansion of certain V beta families was observed in CD4+ and CD8+ T-cells the T-cell receptor V beta subset distribution was similar among CD38+ and CD38-, CD4+ and CD8+ T-cells, suggesting that CD38+ expression was either independent of an HIV-encoded antigen-driven process or rather indicative of T-cell immaturity. It is proposed that the phenotype of circulating CD4+ and CD8+ T-cells of HIV-infected patients is a feature of two different mechanisms: (i) an in vitro activation state responsible for increased DR expression and selective expansion of V beta gene-defined subsets, and (ii) T-cell immaturity due to an increased turnover of these cells and accounting for increased CD38 expression.
对42例HIV阳性患者的外周血CD4⁺和CD8⁺T淋巴细胞进行三色自动流式细胞术检测，使用三色抗CD4或抗CD8、藻红蛋白抗CD38和异硫氰酸荧光素抗HLA-DR单克隆抗体，以进一步阐明最近提出的T细胞激活假说，该假说用于解释HIV感染期间观察到的CD4⁺T细胞异常。CD4⁺CD38⁺T细胞构成了HIV感染患者循环CD4⁺T细胞的主要部分，并且随着疾病进展其HLA-DR分子阳性率增加。CD4⁺T细胞上CD38和HLA-DR的表达水平与CD8⁺T细胞的表达水平以及β2-微球蛋白水平呈正相关。接下来，为了确定CD38表达是否与Vβ基因定义的亚群的选择性扩增或缺失相关，我们使用13种针对Vβ家族的单克隆抗体比较了HIV感染的CDC II期患者CD38⁺和CD38⁻T细胞之间的Vβ基因频率。虽然在CD4⁺和CD8⁺T细胞中观察到某些Vβ家族的选择性扩增，但CD38⁺和CD38⁻、CD4⁺和CD8⁺T细胞之间的T细胞受体Vβ亚群分布相似，这表明CD38⁺表达要么独立于HIV编码抗原驱动的过程，要么更表明T细胞不成熟。有人提出，HIV感染患者循环CD4⁺和CD8⁺T细胞的表型是两种不同机制的特征：（i）一种体外激活状态，导致DR表达增加和Vβ基因定义的亚群选择性扩增；（ii）T细胞不成熟，这是由于这些细胞的更新增加，并导致CD38表达增加。
