Gradella Villalva Denise, Diociaiuti Marco, Giansanti Luisa, Petaccia Manuela, Bešker Neva, Mancini Giovanna
Dipartimento di Chimica, Università degli Studi di Roma "Sapienza" , P.le Aldo Moro 5, 00185 Roma, Italy.
Dipartimento di Tecnologia e Salute, Istituto Superiore di Sanità , Viale Regina Elena 299, 00161 Roma, Italy.
J Phys Chem B. 2016 Feb 18;120(6):1126-33. doi: 10.1021/acs.jpcb.5b11836. Epub 2016 Feb 9.
Pyrene lipids are useful tools to investigate membrane organization and intracellular lipid trafficking. The molecular interactions controlling the organization of lipid monolayers composed of a cationic amphiphile tagged with a pyrene residue and a saturated or unsaturated phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine, were investigated by Langmuir trough isotherms to understand how the molecular structure of the components and their relative amount affect the physicochemical properties of lipid monolayers. The obtained results show that the cationic headgroups and unsaturation of hydrophobic chains strongly affect the organization of the lipid monolayer as a function of the amount of components. On the other hand, the presence of the pyrene moiety does not seem to have a marked influence on the interaction within lipid assembly.
芘脂质是研究膜组织和细胞内脂质运输的有用工具。通过Langmuir槽等温线研究了由带有芘残基的阳离子两亲物与饱和或不饱和磷脂（即1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱和1,2-二油酰-sn-甘油-3-磷酸胆碱）组成的脂质单层组织的分子相互作用，以了解组分的分子结构及其相对含量如何影响脂质单层的物理化学性质。所得结果表明，阳离子头基和疏水链的不饱和度作为组分含量的函数强烈影响脂质单层的组织。另一方面，芘部分的存在似乎对脂质组装体中的相互作用没有显著影响。
