Egf17和miRNA-126-5p在有症状颈动脉疾病中的表达
Expression of Egfl7 and miRNA-126-5p in Symptomatic Carotid Artery Disease.
作者信息
Sezer Zhmurov Çiğdem, Timirci-Kahraman Özlem, Amadou Fatimat'ul Zahara, Fazlıoğulları Osman, Başaran Cem, Catal Tunc, Zeybek Ümit, Bermek Hakan
机构信息
1 Department of Molecular Biology and Genetics, Istanbul Technical University , Istanbul, Turkey .
2 Department of Genetics and Bioengineering, Istanbul Bilgi University , Istanbul, Turkey .
出版信息
Genet Test Mol Biomarkers. 2016 Mar;20(3):125-9. doi: 10.1089/gtmb.2015.0252. Epub 2016 Jan 22.
BACKGROUND
Neoangiogenesis inside the atherosclerotic plaques has been linked to progression of the disease. Egfl7, a key player in adult angiogenesis, was found to be upregulated in response to vascular injury in rats. Egfl7 encodes for miR-126-3p and miR-126-5p. Specific information about miRNA-126-5p and its expression in cardiovascular disease is scarce in comparison to that of miR-126-3p.
OBJECTIVES
A gene expression study was conducted to investigate the levels of Egfl7 and miRNA126-5p in human carotid artery atherosclerotic plaques aiming to gain a better understanding of the role of neoangiogenesis within plaques and the mechanisms causing atherosclerosis progression.
METHODS
Egfl7 and miR-126-5p levels were studied in 14 plaque samples and 14 control samples using real-time PCR. The fold change between the carotid artery plaque tissue and control tissue was calculated using the 2(-ΔΔCT) method.
RESULTS
Egfl7 was upregulated in the 11 plaque samples compared to controls, while expression levels of miR-126-5p was higher in eight of the plaque samples and lower in six as compared to control samples. Upregulation of miR-126-5p expression was correlated with high low-density lipoprotein (LDL) cholesterol (p = 0.023).
CONCLUSIONS
Our findings suggest that the upregulation of Egfl7 promotes neoangiogenesis within the plaques, contributing to disease progression.
背景
动脉粥样硬化斑块内的新生血管形成与疾病进展相关。Egfl7是成人血管生成中的关键因子，在大鼠血管损伤时被发现上调。Egfl7编码miR-126-3p和miR-126-5p。与miR-126-3p相比，关于miRNA-126-5p及其在心血管疾病中表达的具体信息较少。
目的
进行一项基因表达研究，以调查人颈动脉粥样硬化斑块中Egfl7和miRNA126-5p的水平，旨在更好地了解斑块内新生血管形成的作用以及导致动脉粥样硬化进展的机制。
方法
使用实时PCR研究了14个斑块样本和14个对照样本中的Egfl7和miR-126-5p水平。采用2(-ΔΔCT)法计算颈动脉斑块组织与对照组织之间的倍数变化。
结果
与对照组相比，11个斑块样本中的Egfl7上调，而8个斑块样本中miR-126-5p的表达水平高于对照组，6个低于对照组。miR-126-5p表达上调与高低密度脂蛋白（LDL）胆固醇相关（p = 0.023）。
结论
我们的研究结果表明，Egfl7的上调促进了斑块内的新生血管形成，导致疾病进展。
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