Gołębiowski Marek, Cerkowniak Magdalena, Ostachowska Aleksandra, Boguś Mieczysława I, Stepnowski Piotr
Laboratory of Analysis of Natural Compounds, Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308, Gdańsk, Poland.
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warszawa, Poland.
Biomed Chromatogr. 2016 Aug;30(8):1318-23. doi: 10.1002/bmc.3688. Epub 2016 Feb 17.
Insects are of growing significance in veterinary medicine and human healthcare; therefore, an understanding of their biology is very important. The cuticular and internal fatty acid compositions of Chorthippus brunneus males and females have been studied for the first time. The lipids of males and females were separated into classes of compounds using high-performance liquid chromatography with a laser light scattering detector. The free fatty acid (FFA) fractions obtained by HPLC were silylated and then analyzed by GC-MS. The cuticular lipids of males contained 15 saturated, four unsaturated with even-numbered and two unsaturated with odd-numbered carbon chains, FFAs ranging from C8 to C25. The major free fatty acids in males were C16 (20.8%), C18:2 (8.5%), C18:1 (32.9%) and C18 (24.4%). The cuticular lipids of females contained 17 saturated, four monounsaturated and two diunsaturated free fatty acids ranging from C8 to C30. The major cuticular fatty acids in females were C16 (25.1%), C18:2 (6.2%), C18:1 (23.7%) and C18:0 (33.2%). The internal FFAs of males consisted of 20 compounds ranging from C8 to C26. Four of these compounds were detected as major compounds: C16 (14.1%), C18:2 (21.6%), C18:1 (38.0%) and C18 (22.5%). Among 18 internal free fatty acids of females, C16 (22.3%), C18:2 (10.9%), C18:1 (40.2%) and C18 (20.5%) were the most abundant compounds. The following cuticular fatty acids present in the lipids of females were absent in the lipids of males: C26, C27 and C30. On the other hand, only C24 was absent from the cuticular lipids of females. Only C10 and C24 internal fatty acids present in the lipids of males were absent in the lipids of females. Copyright © 2016 John Wiley & Sons, Ltd.
昆虫在兽医学和人类医疗保健中的重要性日益凸显；因此，了解它们的生物学特性非常重要。首次对雄性和雌性 brunneus 草螽的表皮和内部脂肪酸组成进行了研究。使用配备激光散射检测器的高效液相色谱法将雄性和雌性的脂质分离为不同的化合物类别。通过高效液相色谱法获得的游离脂肪酸（FFA）馏分进行硅烷化处理，然后通过气相色谱 - 质谱联用仪进行分析。雄性的表皮脂质含有 15 种饱和脂肪酸、4 种偶数碳链的不饱和脂肪酸和 2 种奇数碳链的不饱和脂肪酸，游离脂肪酸范围从 C8 到 C25。雄性中的主要游离脂肪酸为 C16（20.8%）、C18:2（8.5%）、C18:1（32.9%）和 C18（24.4%）。雌性的表皮脂质含有 17 种饱和脂肪酸、4 种单不饱和脂肪酸和 2 种二不饱和脂肪酸，范围从 C8 到 C30。雌性中的主要表皮脂肪酸为 C16（25.1%）、C18:2（6.2%）、C18:1（23.7%）和 C18:0（33.2%）。雄性的内部游离脂肪酸由 20 种化合物组成，范围从 C8 到 C26。其中 4 种化合物被检测为主要化合物：C16（14.1%）、C18:2（21.6%）、C18:1（38.0%）和 C18（22.5%）。在雌性的 18 种内部游离脂肪酸中，C16（22.3%）、C18:2（10.9%）、C18:1（40.2%）和 C18（20.5%）是含量最丰富的化合物。雌性脂质中存在的以下表皮脂肪酸在雄性脂质中不存在：C26、C27 和 C30。另一方面，雌性的表皮脂质中仅不存在 C24。雄性脂质中存在的仅 C10 和 C24 内部脂肪酸在雌性脂质中不存在。版权所有 © 2016 约翰威立父子有限公司。
