Van Bocxlaer J F, Verhaeghe B J, Wauters A E, De Marez W D, Thienpont L M, De Leenheer A P
Laboratoria voor Medische Biochemie en Klinische Analyse, Rijksuniversiteit Gent, Belgium.
Biomed Environ Mass Spectrom. 1989 Aug;18(8):566-71. doi: 10.1002/bms.1200180810.
The determination of pipecolic acid in serum or plasma by positive chemical ionization gas chromatography/mass spectrometry is assessed. This quantitative method involves stable isotope dilution and cation-exchange solid-phase extraction. Several derivatives of pipecolic acid and its octadeuterated analogue were investigated for their mass spectrometric characteristics. The heptafluorobutyric methyl ester derivatives afford optimal resolution on gas chromatography of biological extracts. Moreover, the derivatizing reagent (methanolic HCl) allows a combined elution and derivatization. Selected ion monitoring is performed on the [M + H]+ ions of both analyte and internal standard, at m/z 340 and 348, respectively. Serum or plasma samples from healthy subjects and patients suspected of peroxisomal diseases have been examined.
对采用正化学电离气相色谱/质谱法测定血清或血浆中哌啶酸进行了评估。这种定量方法涉及稳定同位素稀释和阳离子交换固相萃取。研究了哌啶酸及其十八氘代类似物的几种衍生物的质谱特性。七氟丁酸甲酯衍生物在生物提取物的气相色谱分析中提供了最佳分辨率。此外，衍生化试剂（甲醇盐酸）允许洗脱和衍生化同时进行。分别在m/z 340和348处对分析物和内标的[M + H]+离子进行选择离子监测。已对来自健康受试者和疑似过氧化物酶体疾病患者的血清或血浆样本进行了检测。
