Ozaydin Tugba, Sur Emrah, Oznurlu Yasemin, Celik Ilhami, Uluisik Deniz
Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
Department of Physiology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
Microsc Res Tech. 2016 Apr;79(4):251-7. doi: 10.1002/jemt.22624. Epub 2016 Jan 22.
The aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation.
本研究的目的是调查热休克蛋白70（Hsp70）和增殖细胞核抗原（PCNA）在小鼠不同妊娠阶段胎盘组织中的免疫组化分布情况。为此，共使用了18只12 - 14周龄的瑞士白化雌性小鼠。在妊娠第3天（早期）、第10天（中期）和第17天（晚期）处死雌性小鼠，并采集妊娠子宫的着床部位样本。切片横向穿过着床部位的中心区域，用苏木精和伊红染色进行组织学检查。PCNA和Hsp70进行免疫组化染色。由于在妊娠第3天确定的胎盘仍未形成，仅在腔上皮和蜕膜基质细胞中评估Hsp70和PCNA阳性情况。在妊娠第10天和第17天，在胎盘的迷路区、交界区和蜕膜层评估Hsp70和PCNA阳性情况。观察到Hsp70在滋养层细胞和蜕膜细胞中表达，并且在整个妊娠期间相对恒定。该蛋白在滋养层细胞中标记强烈；而蜕膜细胞显示中度染色。在妊娠早期小鼠子宫中，PCNA主要定位于蜕膜基质细胞。在妊娠中期，滋养层细胞和蜕膜细胞显示出高度增殖活性。然而，在妊娠晚期PCNA阳性细胞百分比显著下降。
