用于处理酒糟甲烷化沼气的脱硫系统的宏基因组分析。
Metagenomic analysis of a desulphurisation system used to treat biogas from vinasse methanisation.
机构信息
Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil.
Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil; Methanum Waste and Energy, Alameda do Ingá, 840, Room 908, Vale do Sereno, Nova Lima, Minas Gerais State 34.000-000, Brazil.
出版信息
Bioresour Technol. 2016 Apr;205:58-66. doi: 10.1016/j.biortech.2016.01.007. Epub 2016 Jan 8.
We investigated the response of microbial community to changes in H2S loading rate in a microaerated desulphurisation system treating biogas from vinasse methanisation. H2S removal efficiency was high, and both COD and DO seemed to be important parameters to biomass activity. DGGE analysis retrieved sequences of sulphide-oxidising bacteria (SOB), such as Thioalkalimicrobium sp. Deep sequencing analysis revealed that the microbial community was complex and remained constant throughout the experiment. Most sequences belonged to Firmicutes and Proteobacteria, and, to a lesser extent, Bacteroidetes, Chloroflexi, and Synergistetes. Despite the high sulphide removal efficiency, the abundance of the taxa of SOB was low, and was negatively affected by the high sulphide loading rate.
我们研究了在微需氧脱硫系统中，H2S 加载速率变化对微生物群落的响应，该系统处理来自酒糟沼气甲烷化的沼气。H2S 去除效率很高，COD 和 DO 似乎都是生物量活性的重要参数。DGGE 分析检索到硫氧化细菌（SOB）的序列，如硫代碱微菌属。深度测序分析表明，微生物群落复杂且在整个实验过程中保持不变。大多数序列属于 Firmicutes 和 Proteobacteria，其次是 Bacteroidetes、Chloroflexi 和 Synergistetes。尽管 H2S 去除效率很高，但 SOB 类群的丰度较低，且受到高 H2S 加载速率的负面影响。
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