Revuelta M, Arteaga O, Alvarez A, Martinez-Ibargüen A, Hilario E
Department of Cell Biology and Histology, School of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Barrio Sarriena s/n, Leioa, E-48940, Vizcaya, Spain.
Department of Otorhinolaryngology, School of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, E-48940, Vizcaya, Spain.
Mol Neurobiol. 2017 Mar;54(2):1129-1143. doi: 10.1007/s12035-016-9724-6. Epub 2016 Jan 25.
The perinatal brainstem is known to be very vulnerable to hypoxic-ischemic events which can lead to deafness, swallowing dysfunction, and defective respiratory control. The aim of the present work was to evaluate the potential neuroprotective effects of nicotine, melatonin, resveratrol, and docosahexaenoic acid on the expression of a panel of genes in the brainstem following hypoxic-ischemic damage. Quantitative PCR was used to examine gene expression 3 and 12 h after the damage, and immunohistochemistry was employed to evaluate neurons, astrocytes, and synaptic vesicles 24 h post insult. We found that the expression of some immediate-early genes, as well as that of inflammatory genes TNF-α, COX2, and caspase 3, was upregulated in response to the insult. Twenty-four hours after the damage, the percentage of NeuN and synaptophysin immunolabeled cells was found to be reduced while GFAP expression was upregulated. No differences were observed in ROS gene expression following treatments.
已知围产期脑干对缺氧缺血事件非常敏感，这些事件可导致耳聋、吞咽功能障碍和呼吸控制缺陷。本研究的目的是评估尼古丁、褪黑素、白藜芦醇和二十二碳六烯酸对缺氧缺血性损伤后脑干中一组基因表达的潜在神经保护作用。在损伤后3小时和12小时使用定量PCR检测基因表达，并在损伤后24小时采用免疫组织化学评估神经元、星形胶质细胞和突触小泡。我们发现，一些即早基因以及炎症基因TNF-α、COX2和caspase 3的表达因损伤而上调。损伤后24小时，发现NeuN和突触素免疫标记细胞的百分比降低，而GFAP表达上调。治疗后ROS基因表达未观察到差异。
