脱氧雪腐镰刀菌烯醇对体外培养的伴刀豆球蛋白A刺激的鸡脾淋巴细胞钙稳态的影响。

Effects of deoxynivalenol on calcium homeostasis of concanavalin A--Stimulated splenic lymphocytes of chickens in vitro.

作者信息

Ren Zhihua, Wang Yachao, Deng Huidan, Deng Youtian, Deng Junliang, Zuo Zhicai, Wang Ya, Peng Xi, Cui Hengmin, Shen Liuhong, Yu Shumin, Cao Suizhong

机构信息

College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.

出版信息

Exp Toxicol Pathol. 2016 Apr;68(4):241-5. doi: 10.1016/j.etp.2016.01.001. Epub 2016 Jan 19.

DOI:10.1016/j.etp.2016.01.001

PMID:26809658

Abstract

In this study, the in vitro effects of the treatment of concanavalin A (Con A)--stimulated splenic lymphocytes with DON were examined. Splenic lymphocytes isolated from chickens were stimulated with 12.5 μg/mL Con A and exposed to deoxynivalenol (DON) (0-50 μg/mL) for 48 h. The intracellular calcium concentration ([Ca(2+)]i), pH, calmodulin (CaM) mRNA levels, and Na(+),K(+)-ATPase and Ca(2+)-ATPase activities were detected. With the DON exposure concentrations increased, the [Ca(2+)]i and CaM mRNA levels gradually increased in a dose-dependent manner, and all the evaluated conconcentrations affected ATPase activity to the same extent. There were significant differences (P<0.05 or P<0.01) between the treatment groups and the control group. These results indicate that an imbalance in calcium homeostasis and intracellular acidification are components of DON cytotoxicity in chicken lymphocytes.

摘要

在本研究中，检测了脱氧雪腐镰刀菌烯醇（DON）处理伴刀豆球蛋白A（Con A）刺激的脾淋巴细胞的体外效应。从鸡中分离的脾淋巴细胞用12.5μg/mL Con A刺激，并暴露于脱氧雪腐镰刀菌烯醇（DON）（0 - 50μg/mL）中48小时。检测细胞内钙浓度（[Ca(2+)]i）、pH、钙调蛋白（CaM）mRNA水平以及Na(+)、K(+)-ATP酶和Ca(2+)-ATP酶活性。随着DON暴露浓度增加，[Ca(2+)]i和CaM mRNA水平以剂量依赖性方式逐渐增加，并且所有评估浓度对ATP酶活性的影响程度相同。处理组与对照组之间存在显著差异（P<0.05或P<0.01）。这些结果表明，钙稳态失衡和细胞内酸化是DON对鸡淋巴细胞细胞毒性的组成部分。