Yun Jeonghee, Lee Sang Don, Cho Kyung-Suk
Department of Environmental Science and Engineering, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 120-750, Republic of Korea.
Appl Microbiol Biotechnol. 2016 May;100(10):4675-83. doi: 10.1007/s00253-016-7314-1. Epub 2016 Jan 25.
This study aimed to investigate the interaction between methane production performance and active microbial community dynamics at different loading rates by increasing influent substrate concentration. The model system was an upflow anaerobic sludge blanket (UASB) reactor using molasses wastewater. The active microbial community was analyzed using a ribosomal RNA-based approach in order to reflect active members in the UASB system. The methane production rate (MPR) increased with an increase in organic loading rate (OLR) from 3.6 to 5.5 g COD·L(-1)·day(-1) and then it decreased with further OLR addition until 9.7 g COD·L(-1)·day(-1). The UASB reactor achieved a maximum methane production rate of 0.48 L·L(-1)·day(-1) with a chemical oxygen demand (COD) removal efficiency of 91.2 % at an influent molasses concentration of 16 g COD·L(-1) (OLR of 5.5 g COD·L(-1)·day(-1)). In the archaeal community, Methanosarcina was predominant irrespective of loading rate, and the relative abundance of Methanosaeta increased with loading rate. In the bacterial community, Firmicutes and Eubacteriaceae were relatively abundant in the loading conditions tested. The network analysis between operation parameters and microbial community indicated that MPR was positively associated with most methanogenic archaea, including the relatively abundant Methanosarcina and Methanosaeta, except Methanofollis. The most abundant Methanosarcina was negatively associated with Bifidobacterium and Methanosaeta, whereas Methanosaeta was positively associated with Bifidobacterium.
本研究旨在通过提高进水底物浓度，研究不同负荷率下甲烷产生性能与活性微生物群落动态之间的相互作用。模型系统是一个使用糖蜜废水的上流式厌氧污泥床（UASB）反应器。为了反映UASB系统中的活性成员，采用基于核糖体RNA的方法对活性微生物群落进行了分析。甲烷产生率（MPR）随着有机负荷率（OLR）从3.6增加到5.5 g COD·L⁻¹·d⁻¹而增加，然后随着OLR进一步增加直至9.7 g COD·L⁻¹·d⁻¹而降低。在进水糖蜜浓度为16 g COD·L⁻¹（OLR为5.5 g COD·L⁻¹·d⁻¹）时，UASB反应器实现了最大甲烷产生率0.48 L·L⁻¹·d⁻¹，化学需氧量（COD）去除效率为91.2%。在古菌群落中，无论负荷率如何，甲烷八叠球菌均占主导地位，而甲烷鬃菌的相对丰度随负荷率增加。在细菌群落中，在所测试的负荷条件下，厚壁菌门和真杆菌科相对丰富。操作参数与微生物群落之间的网络分析表明，MPR与大多数产甲烷古菌呈正相关，包括相对丰富的甲烷八叠球菌和甲烷鬃菌，但不包括甲烷泡菌属。最丰富的甲烷八叠球菌与双歧杆菌和甲烷鬃菌呈负相关，而甲烷鬃菌与双歧杆菌呈正相关。
