连续极低频磁场对大鼠中缝核5-羟吲哚乙酸水平的影响。

The effect of continuous ELF-MFs on the level of 5-HIAA in the raphe nucleus of the rat.

作者信息

Shahbazi-Gahrouei Daryoush, Shiri Leila, Alaei Hojjatollah, Naghdi Naser

机构信息

Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

J Radiat Res. 2016 Mar;57(2):127-32. doi: 10.1093/jrr/rrv093. Epub 2016 Jan 24.

DOI:10.1093/jrr/rrv093

PMID:26811259

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4795953/

Abstract

The aim of this study was to investigate the effect of continuous extremely low frequency magnetic fields (ELF-MFs) with a frequency of 10 Hz and an intensity of 690-720 μT on the level of 5-hydroxyindolacetic acid (5-HIAA) in adult male Wistar rats. A total of 24 adult Wistar male rats were used, and after exposure with an ELF-MF for 15 successive days, all rats in each test were anesthetized with chloral hydrate. Then, they were placed in a stereotaxic frame for surgery and a microdialysis process. Dialysate samples were analyzed to measure the amount of 5-HIAA by high performance liquid chromatography (HPLC) using electrochemical detection. Results showed that ELF-MF exposure for 15 days, 1 h daily, was not effective in altering the level of 5-HIAA. However, ELF-MF exposure for 15 days, 3 h daily, decreased the level of the 5-HIAA in the raphe nucleus. It can be concluded that ELF-MFs affect the serotonergic system and may be used to treat nervous system diseases. This study is an initial step towards helping cure depression using ELF-MFs.

摘要

本研究的目的是调查频率为10Hz、强度为690 - 720μT的连续极低频磁场（ELF - MFs）对成年雄性Wistar大鼠5 - 羟吲哚乙酸（5 - HIAA）水平的影响。总共使用了24只成年Wistar雄性大鼠，在连续15天暴露于ELF - MF后，每个试验组的所有大鼠均用氯水合醛麻醉。然后，将它们置于立体定位框架中进行手术和微透析过程。使用电化学检测的高效液相色谱法（HPLC）分析透析液样本以测量5 - HIAA的量。结果表明，每天1小时、连续15天暴露于ELF - MF对改变5 - HIAA水平无效。然而，每天3小时、连续15天暴露于ELF - MF会降低中缝核中5 - HIAA的水平。可以得出结论，ELF - MFs影响血清素能系统，可用于治疗神经系统疾病。本研究是使用ELF - MFs帮助治疗抑郁症的第一步。