Chen Chun-Hsiung, Chen Hung-An, Liao Hsien-Tzung, Liu Chin-Hsiu, Tsai Chang-Youh, Chou Chung-Tei
Division of Allergy, Immunology and Rheumatology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taiwan, and School of Medicine, Tzu Chi University, Hualien; and Taipei Medial University, and Municipal Wan Fang Hospital, Taipei, Taiwan.
Chi Mei Medical Center, Tainan, Taiwan.
Clin Exp Rheumatol. 2016 Jan-Feb;34(1):100-5. Epub 2016 Jan 20.
To investigate the suppressors of cytokine signalling (SOCS1 and SOCS3) expression in peripheral blood cells in ankylosing spondylitis (AS), and their associations with clinical and laboratory manifestations.
The levels of SOCS1 and SOCS3 mRNA in peripheral blood mononuclear cells (PBMCs), T cells and monocytes were measured by RT-PCR in 53 AS patients and 31 healthy controls. Patient's serum IL-6, IL-10 and IL-17A levels were determined by ELISA. We evaluated patient's disease activity, functional ability and global assessment, and tested their ESR, CRP and IgA levels.
Cellular SOCS1 expression did not show significant differences between AS patients and controls. However, T cells SOCS1 decreased significantly in the AS subgroup with lower ESR than controls (p=0.013). PBMCs (p=0.047) and T cells (p=0.035) SOCS1 decreased significantly in the AS subgroup with lower CRP than controls. Importantly, SOCS3 expression increased significantly in AS patients compared to the controls in PBMCs (p=0.025), T cells (p=0.003) and monocytes (p=0.009). Moreover, PBMCs SOCS3 correlated with ESR (r=0.297, p=0.031) and CRP (r=0.320, p=0.019). T cells SOCS3 correlated with BASFI (r=0.337, p=0.015), ESR (r=0.435, p=0.001) and CRP (r=0.300, p=0.029). Monocytes SOCS3 correlated with ESR (r=0.281, p=0.041) and IgA (r=0.426, p=0.006). Furthermore, T cells SOCS1 (r=-0.454, p=0.023) and T cells SOCS3 (r=-0.405, p=0.045) negatively correlated with serum IL-17A. Monocytes SOCS3 negatively correlated with serum IL-6 (r=-0.584, p=0.002).
The decreased SOCS1 and increased SOCS3 expression in AS PBMCs and T cells, and their correlation with patient's functional ability, acute-phase reactants and serum pro-inflammatory cytokines suggested that SOCS may participate in the pathogenesis of AS.
研究强直性脊柱炎（AS）患者外周血细胞中细胞因子信号转导抑制因子（SOCS1和SOCS3）的表达情况及其与临床和实验室表现的相关性。
采用逆转录聚合酶链反应（RT-PCR）检测53例AS患者和31例健康对照者外周血单个核细胞（PBMC）、T细胞和单核细胞中SOCS1和SOCS3 mRNA的水平。采用酶联免疫吸附测定（ELISA）法检测患者血清白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和白细胞介素-17A（IL-17A）水平。评估患者的疾病活动度、功能能力和整体评估情况，并检测其红细胞沉降率（ESR）、C反应蛋白（CRP）和免疫球蛋白A（IgA）水平。
AS患者与对照组之间细胞SOCS1表达无显著差异。然而，ESR低于对照组的AS亚组中T细胞SOCS1显著降低（p=0.013）。CRP低于对照组的AS亚组中PBMC（p=0.047）和T细胞（p=0.035）SOCS1显著降低。重要的是，与对照组相比，AS患者PBMC（p=0.025）、T细胞（p=0.003）和单核细胞（p=0.009）中SOCS3表达显著增加。此外，PBMC中SOCS3与ESR（r=0.297，p=0.031）和CRP（r=0.320，p=0.019）相关。T细胞SOCS3与巴斯强直性脊柱炎功能指数（BASFI）（r=0.337，p=0.015）、ESR（r=0.435，p=0.001）和CRP（r=0.300，p=0.029）相关。单核细胞SOCS3与ESR（r=0.281，p=0.041）和IgA（r=0.426，p=0.006）相关。此外，T细胞SOCS1（r=-0.454，p=0.023）和T细胞SOCS3（r=-0.405，p=0.045）与血清IL-17A呈负相关。单核细胞SOCS3与血清IL-6呈负相关（r=-0.584，p=0.002）。
AS患者PBMC和T细胞中SOCS1表达降低，SOCS3表达增加，且它们与患者的功能能力、急性期反应物和血清促炎细胞因子相关，提示SOCS可能参与AS的发病机制。
