妊娠糖尿病女性自然杀伤细胞上自然细胞毒性受体的表达及细胞内细胞因子的产生
Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus.
作者信息
Chiba Hitomi, Fukui Atsushi, Fuchinoue Kohei, Funamizu Ayano, Tanaka Kanji, Mizunuma Hideki
机构信息
Department of Obstetrics and Gynecology, Graduate School of Medicine, Hirosaki University, Hirosaki, Aomori, Japan.
出版信息
Am J Reprod Immunol. 2016 May;75(5):529-38. doi: 10.1111/aji.12491. Epub 2016 Jan 27.
PROBLEM
To determine the role of peripheral blood NK (pNK) cells in putative etiology of gestational diabetes, the expression of surface markers on pNK cells and the percentage of cytokine-producing pNK cells in women at 12 weeks of pregnancy with gestational diabetes mellitus (GDM) were studied.
METHOD OF STUDY
Multicolor flow cytometry was used to analyze the expression of NK cell surface receptors (CD16, NKp46, and NKp30) and intracellular cytokines (IFN-γ, TNF-α, TGF-β, and VEGF) in pNK cells (CD56(dim) and CD56(bright) ) at 12 weeks of pregnancy with GDM (n = 7) and non-GDM (n = 28).
RESULTS
CD56(bright) /CD16(-) NK and CD56(bright) /NKp46(+) NK cell percentage were significantly lower in GDM women than that in non-GDM women. IFN-γ- and TNF-α-producing CD56(+) cells, respectively, were significantly high, while TGF-β- and VEGF-producing CD56(+) cells and CD56(bright) cells, respectively, were significantly low in GDM women.
CONCLUSIONS
Women with GDM possibly have abnormal NK cell function for the expression of surface receptors and cytokine production.
问题
为了确定外周血自然杀伤（pNK）细胞在妊娠期糖尿病可能的病因学中的作用，研究了患有妊娠期糖尿病（GDM）的孕妇在妊娠12周时pNK细胞表面标志物的表达以及产生细胞因子的pNK细胞的百分比。
研究方法
采用多色流式细胞术分析患有GDM（n = 7）和非GDM（n = 28）的孕妇在妊娠12周时pNK细胞（CD56（dim）和CD56（bright））中自然杀伤细胞表面受体（CD16、NKp46和NKp30）和细胞内细胞因子（IFN-γ、TNF-α、TGF-β和VEGF）的表达。
结果
GDM女性中CD56（bright）/CD16（-）自然杀伤细胞和CD56（bright）/NKp46（+）自然杀伤细胞的百分比显著低于非GDM女性。GDM女性中产生IFN-γ和TNF-α的CD56（+）细胞分别显著增多，而产生TGF-β和VEGF的CD56（+）细胞和CD56（bright）细胞分别显著减少。
结论
患有GDM的女性可能因表面受体的表达和细胞因子的产生而具有异常的自然杀伤细胞功能。
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