Quan Yingjun, Xu Ming, Cui Peng, Ye Min, Zhuang Biao, Min Zhijun
Department of General Surgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China.
Department of General Surgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China; Email:
Zhonghua Zhong Liu Za Zhi. 2015 Oct;37(10):764-8.
The purpose of this study was to detect the expression of GRHL2 in colorectal cancer (CRC) tissues, and to assess the relationship between GRHL2 expression and clinicopathological features.
Immunohistochemistry was used to examine GRHL2 in 75 CRC tissues. GRHL2 mRNA and protein levels in the CRC tissues were also analyzed by qRT-PCR and Western blot. The relationship between GRHL2 and clinicopathological features was assessed by Pearson's chi-square (χ(2)) test.
Positive immunoreactivity for GRHL2 was detected in the nuclei of CRC cells. GRHL2 expression was increased in CRC tissues compared withthat in the paired non-tumor tissues (61.3% vs. 44.0%, P<0.01). Moreover, qRT-PCR results showed that the relative expression level of GRHL2 mRNA in the colorectal cancer tissue was (2.64±0.35), significantly higher than that of normal mucosa tissue (1.19±0.23, P<0.001). The expression level of GRHL2 mRNA was higher in stage III-IV patients (2.84±0.36) than that of stage I-II cases (2.31±0.32, P<0.05). Western blot results also showed that the expression level of GRHL2 protein in the colorectal carcinoma tissue was significantly higher than that in the normal mucosa (P<0.05). GRHL2 expression was positively correlated with tumor size, TNM stage and Ki-67 (P<0.05, respectively).
Taking together, our findings demonstrate that GRHL2 is overexpressed in CRC, and plays an important role in the progression of CRC.
本研究旨在检测结直肠癌（CRC）组织中GRHL2的表达，并评估GRHL2表达与临床病理特征之间的关系。
采用免疫组织化学法检测75例CRC组织中的GRHL2。同时通过qRT-PCR和蛋白质免疫印迹法分析CRC组织中GRHL2 mRNA和蛋白水平。采用Pearson卡方检验评估GRHL2与临床病理特征之间的关系。
在CRC细胞核中检测到GRHL2阳性免疫反应。与配对的非肿瘤组织相比，CRC组织中GRHL2表达增加（61.3%对44.0%，P<0.01）。此外，qRT-PCR结果显示，结直肠癌组织中GRHL2 mRNA的相对表达水平为（2.64±0.35），显著高于正常黏膜组织（1.19±0.23，P<0.001）。III-IV期患者GRHL2 mRNA表达水平（2.84±0.36）高于I-II期患者（2.31±0.32，P<0.05）。蛋白质免疫印迹结果也显示，结直肠癌组织中GRHL2蛋白表达水平显著高于正常黏膜组织（P<0.05）。GRHL2表达与肿瘤大小、TNM分期和Ki-67呈正相关（分别为P<0.05）。
综上所述，我们的研究结果表明GRHL2在CRC中过表达，并在CRC进展中起重要作用。
