Thakur Rameshwari, Goyal Roma
Associate Professor, Department of Microbiology, Muzaffarnagar Medical College , Meerut Road, Muzaffarnagar, UP, India .
Demonstrator, Department of Microbiology, Muzaffarnagar Medical College , Meerut Road, Muzaffarnagar, UP, India .
J Clin Diagn Res. 2015 Dec;9(12):WD03-4. doi: 10.7860/JCDR/2015/14488.6859. Epub 2015 Dec 1.
Tinea capitis is a common dermatophyte infection affecting scalp and hair of pre-pubertal children. With introduction of Griseofulvin in 1958, there has been considerable improvement in the treatment of tinea capitis. A seven-year-old male child was brought to the dermatology clinic. He presented with diffuse white scaly patches of alopecia on scalp of one-year duration. The child was sent to the microbiology section of the National Health laboratory, Botswana for the collection of the samples. The samples consisted of scalp scrapings and few plucked hairs from the suspicious areas, which were collected by swab and scalpel blade methods. Potassium hydroxide (10% KOH) mounts were prepared for scales and hair samples. Scales were positive for fungal elements and endothrix type of perforation was seen in hair. Cultures on Sabouraud's dextrose agar (SDA) and Derm agar were incubated at 25°C, which yielded white variant of Trichophyton violaceum after two weeks of incubation. T. vioaceum (white variant) grew in all the plates. After six weeks of treatment with griseofulvin, the repeat culture grew only T. violaceum (violet strain). The child showed a definite clinical improvement.
头癣是一种常见的皮肤癣菌感染，影响青春期前儿童的头皮和头发。自1958年灰黄霉素问世以来，头癣的治疗有了显著改善。一名7岁男童被带到皮肤科诊所。他头皮上出现弥漫性白色鳞屑性脱发斑，病程长达一年。该儿童被送往博茨瓦纳国家卫生实验室的微生物科采集样本。样本包括头皮刮屑和从可疑区域拔下的少量毛发，通过棉签和手术刀方法采集。对鳞屑和毛发样本制备了氢氧化钾（10% KOH）涂片。鳞屑中真菌成分呈阳性，毛发中可见发内型穿孔。在沙氏葡萄糖琼脂（SDA）和皮肤真菌琼脂上培养，于25°C孵育，孵育两周后产生紫色毛癣菌白色变种。所有平板上均生长出紫色毛癣菌（白色变种）。用灰黄霉素治疗六周后，重复培养仅生长出紫色毛癣菌（紫色菌株）。患儿临床症状明显改善。
