Ponelies N, Gosenca D, Ising N, Schoen M, Ruppel K, Vollmar B, Obertacke U
Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Institute for Experimental Surgery, University of Rostock, Schillingallee, 18055, Rostock, Germany.
Eur J Trauma Emerg Surg. 2011 Dec;37(6):645-54. doi: 10.1007/s00068-011-0083-8. Epub 2011 Feb 22.
Previous studies have suggested that an increased catabolic stage of skeletal muscle in pathological situations is mainly a reflection of ubiquitin-proteasome system-controlled proteolysis. The proteolytic mechanisms that occur after local muscle trauma are poorly defined. We investigated the effects of closed soft-tissue trauma on ubiquitin-proteasome dependent protein breakdown in rats (n = 25). The enzymatic activities of the ubiquitination and proteasome reactions were both reduced (p < 0.05) immediately after contusion of the hind limb musculus extensor digitorum longus. The same effect was observed in extracts of lung tissue from the injured animals. Cellular levels of free and protein-conjugated ubiquitin were significantly elevated upon decreased proteolytic activity. Our data support an early-state anti-proteolytic role of the ubiquitin-proteasome pathway after local injury. This further implies that there is a yet-to-be elucidated complex regulatory mechanism of muscle regeneration that involves various proteolytic systems.
先前的研究表明，在病理情况下骨骼肌分解代谢阶段的增加主要反映了泛素 - 蛋白酶体系统控制的蛋白水解。局部肌肉创伤后发生的蛋白水解机制尚不清楚。我们研究了闭合性软组织创伤对大鼠（n = 25）中泛素 - 蛋白酶体依赖性蛋白质分解的影响。后肢趾长伸肌挫伤后，泛素化和蛋白酶体反应的酶活性立即降低（p < 0.05）。在受伤动物的肺组织提取物中也观察到了同样的效果。蛋白水解活性降低时，游离和蛋白质结合泛素的细胞水平显著升高。我们的数据支持局部损伤后泛素 - 蛋白酶体途径的早期抗蛋白水解作用。这进一步意味着存在一种尚未阐明的涉及各种蛋白水解系统的肌肉再生复杂调节机制。
