Ma Xiao-Pin, Yu Guopeng, Chen Xubo, Xiao Qianyi, Shi Zhuqing, Zhang Lu-Yao, Chen Haitao, Zhang Pengyin, Ding Dong-Lin, Huang Hui-Xing, Saiyin Hexige, Chen Tao-Yang, Lu Pei-Xin, Wang Neng-Jin, Yu Hongjie, Conran Carly, Sun Jielin, Zheng S Lilly, Xu Jianfeng, Yu Long, Jiang De-Ke
State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Songhu Rd., Shanghai, 200438, China.
Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.
Tumour Biol. 2016 Jul;37(7):9931-42. doi: 10.1007/s13277-016-4897-1. Epub 2016 Jan 27.
Single nucleotide polymorphisms (SNPs) within microRNAs (miRNAs) are considered potential markers for risk and prognosis of various cancers. In the current study, we aimed to determine whether miR-608 rs4919510 affected hepatocellular carcinoma (HCC) prognosis. We genotyped rs4919510 using DNA from blood samples of 362 HCC patients receiving surgical resection of HCC tumor. Associations between rs4919510 and overall survival (OS) and demographic characteristics and clinical features were estimated using the Cox proportional hazards model. Results showed that HCC patients who carried the rs4919510 CC genotype had a significantly longer OS compared to those who carried the GG genotype (P = 0.013, hazard ratio [HR] = 0.600, 95 % confidence interval [CI] 0.402-0.897) and the CG + GG genotype (P = 0.033, HR = 0.681, 95 % CI 0.479-0.970) in univariate analysis. Similar results were obtained in multivariate analysis. Further stratification analysis indicated that rs4919510 was significantly associated with OS in patients who were satisfied with one of the following criteria: male gender, HbsAg-positive, α-fetoprotein (AFP)-positive, tumor size >5 cm, cirrhosis, solitary tumor, I + II pTNM stage, or no tumor capsule. Finally, a significantly higher frequency of rs4919510 CC genotype was observed in patients with cirrhosis (22.9 %, 55/240) than those without cirrhosis (14.0 %, 17/121) (P = 0.047). In conclusion, our results illustrated the potential role of miR-608 rs4919510 as a prognostic marker for HCC patients undergoing surgical resection of the tumor.
微小RNA（miRNA）中的单核苷酸多态性（SNP）被认为是各种癌症风险和预后的潜在标志物。在本研究中，我们旨在确定miR-608 rs4919510是否影响肝细胞癌（HCC）的预后。我们使用接受HCC肿瘤手术切除的362例HCC患者血样中的DNA对rs4919510进行基因分型。使用Cox比例风险模型评估rs4919510与总生存期（OS）、人口统计学特征和临床特征之间的关联。结果显示，携带rs4919510 CC基因型的HCC患者与携带GG基因型的患者相比，OS显著更长（P = 0.013，风险比[HR]=0.600，95%置信区间[CI] 0.402-0.897），与携带CG + GG基因型的患者相比也显著更长（P = 0.033，HR = 0.681，95%CI 0.479-0.970），单因素分析结果如此。多因素分析也得到了类似结果。进一步的分层分析表明，rs4919510与满足以下任一标准的患者的OS显著相关：男性、乙肝表面抗原（HbsAg）阳性、甲胎蛋白（AFP）阳性、肿瘤大小>5 cm、肝硬化、孤立肿瘤、I + II期pTNM分期或无肿瘤包膜。最后，在有肝硬化的患者中观察到rs4919510 CC基因型的频率（22.9%，55/240）显著高于无肝硬化的患者（14.0%，17/121）（P = 0.047）。总之，我们的结果说明了miR-608 rs4919510作为接受肿瘤手术切除的HCC患者预后标志物的潜在作用。
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