Smith R D, Carr A, Dakin S G, Snelling S J, Yapp C, Hakimi O
The Botnar Research Centre, University of Oxford, Nuffield Orthopaedic Centre, Windmill Road, Oxford, OX3 7LD,
Eur Cell Mater. 2016 Jan 27;31:107-18. doi: 10.22203/ecm.v031a08.
Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds have been developed to augment the repair. Understanding the interaction between patients' cells and scaffolds is important for improving scaffold performance and tendon healing. In this in vitro study, we investigated the response of patient-derived tenocytes to eight different scaffolds. Tested scaffolds included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, and Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, and morphology were assessed. After one day, more cells attached to synthetic scaffolds with dense, fine and aligned fibres (X-Repair and Poly-Tape). Despite low initial cell attachment, the human dermal scaffold (GraftJacket) promoted the greatest proliferation of cells over 13 days. Expression of collagen types I and III were upregulated in cells grown on non-cross-linked porcine dermis (Conexa). Interestingly, the ratio of collagen I to collagen III mRNA was lower on all dermal scaffolds compared to synthetic and biosynthetic scaffolds. These findings demonstrate significant differences in the response of patient-derived tendon cells to scaffolds that are routinely used for rotator cuff surgery. Synthetic scaffolds promoted increased cell adhesion and a tendon-like cellular phenotype, while biological scaffolds promoted cell proliferation and expression of collagen genes. However, no single scaffold was superior. Our results may help understand the way that patients' cells interact with scaffolds and guide the development of new scaffolds in the future.
肩袖撕裂的手术修复具有较高的再撕裂率，因此人们开发了许多支架来加强修复。了解患者细胞与支架之间的相互作用对于提高支架性能和肌腱愈合至关重要。在这项体外研究中，我们调查了患者来源的肌腱细胞对八种不同支架的反应。测试的支架包括X-Repair、Poly-Tape、LARS韧带、生物纤维（合成支架）、生物纤维-CM（生物合成支架）、GraftJacket、Permacol和Conexa（生物支架）。评估了细胞附着、增殖、基因表达和形态。一天后，更多的细胞附着在具有密集、精细且排列整齐纤维的合成支架上（X-Repair和Poly-Tape）。尽管初始细胞附着率较低，但人真皮支架（GraftJacket）在13天内促进了细胞的最大增殖。在非交联猪真皮（Conexa）上生长的细胞中，I型和III型胶原蛋白的表达上调。有趣的是，与合成支架和生物合成支架相比，所有真皮支架上胶原蛋白I与胶原蛋白III mRNA的比例都较低。这些发现表明，患者来源的肌腱细胞对常用于肩袖手术的支架的反应存在显著差异。合成支架促进了细胞粘附增加和肌腱样细胞表型，而生物支架促进了细胞增殖和胶原蛋白基因的表达。然而，没有一种支架是优越的。我们的结果可能有助于理解患者细胞与支架相互作用的方式，并指导未来新型支架的开发。
