Sylovski P, Shopova V, Markova M, Nechev K, Grozeva M
J Toxicol Clin Exp. 1989 Mar-Apr;9(2):67-76.
Biochemical, cytological and morphological studies in Wistar male rats. For N-hexane inhalation treatment, dynamic exposure chambers maintaining a concentration of 5,500 mg/m3 over 5 hours per day were used for 8 days. Immediately there after, the animals were given a single whole-body exposure to 4 Gy X-rays. Bronchoalveolar lavage fluid (BALF) was obtained from removed lungs. Lung homogenates were prepared subsequent to intracapillary lung perfusion via the right cardiac ventricle. Short-term n-hexane inhalation treatment was found to increase BALF total cell counts, predominantly alveolar macrophages (AM); elevated activities in lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) evidenced injury affecting type I and type II pneumocytes over early post-treatment times. Whole-body irradiation alone moderately decreased AM numbers in respiratory pathways. Exposure to both agents combined resulted in depressed activity of a major antioxidant enzyme, superoxide dismutase, and diminished contents of nonprotein sulfhydryl groups in the lungs. Most of the endpoints recorded underwent greater change in the case of combined treatment, indicating synergistic action of n-hexane and ionizing radiation with regard to the biological effects studied.
对雄性Wistar大鼠进行生物化学、细胞学和形态学研究。对于正己烷吸入治疗，使用动态暴露舱，每天5小时维持浓度为5500毫克/立方米，持续8天。此后立即对动物进行单次全身4 Gy X射线照射。从取出的肺中获取支气管肺泡灌洗液（BALF）。通过右心室进行肺内毛细血管灌注后制备肺匀浆。发现短期正己烷吸入治疗会增加BALF总细胞计数，主要是肺泡巨噬细胞（AM）；乳酸脱氢酶（LDH）和碱性磷酸酶（ALP）活性升高表明在治疗后早期I型和II型肺细胞受到损伤。单独全身照射会适度减少呼吸道中的AM数量。同时暴露于两种因素会导致主要抗氧化酶超氧化物歧化酶的活性降低，以及肺中非蛋白巯基含量减少。在联合治疗的情况下，记录的大多数终点变化更大，表明正己烷和电离辐射在所研究的生物学效应方面具有协同作用。
