Panomket Pawana, Wongsana Parichart, Wanram Surasak, Wongratanacheewin Surasakdi, Bartpho Thanatchaporn
J Med Assoc Thai. 2016 Jan;99 Suppl 1:S1-6.
Burkholderia pseudomallei are the causative agents of melioidosis, a disease that has a high relapse rate in endemic areas. The mechanism of relapse is unclear
This study aimed to establish relapsed melioidosis in C57BL/6 mice by induction with B. pseudomallei.
Low doses of B. pseudomallei H777 and its biofilm defective mutant (M10) were intra-gastric fed to C57BL/6 mice. All the infected mice had suppressed immune status by intra-peritoneal injection of hydrocortisone at 2.5 mg per mouse at day 60 post-infection. Inflammatory response to the infection was investigated by histo-pathological studies and monitoring bacterial counts in the blood and organs.
All the infected mice were found to have a high infiltration of mononuclear cells at day 60 post-infection. The results showed high bacterial counts in the blood in both strains post-suppressed immune status after two days. The biofilm mutant and wild type strains produced relapse in C57BL/6 mice but the latter was responsible for significantly more severe inflammation than the biofilm mutant.
Low immune status may cause relapsed melioidosis in hosts with chronic inflammation.
伯克霍尔德菌是类鼻疽的病原体，这种疾病在流行地区的复发率很高。复发机制尚不清楚。
本研究旨在通过用伯克霍尔德菌诱导，在C57BL/6小鼠中建立复发性类鼻疽。
将低剂量的伯克霍尔德菌H777及其生物膜缺陷突变体（M10）经胃内给予C57BL/6小鼠。在感染后第60天，通过腹腔注射氢化可的松（每只小鼠2.5毫克）使所有感染小鼠的免疫状态受到抑制。通过组织病理学研究以及监测血液和器官中的细菌计数来研究对感染的炎症反应。
在感染后第60天，所有感染小鼠均出现单核细胞高度浸润。结果显示，在免疫状态受到抑制两天后，两种菌株的血液中细菌计数都很高。生物膜突变体和野生型菌株在C57BL/6小鼠中均导致复发，但野生型菌株引起的炎症比生物膜突变体严重得多。
低免疫状态可能导致慢性炎症宿主发生复发性类鼻疽。
