Zhao Guomin, Yin Jinshu, Peng Hong, Wang Jing
a Department of Otolaryngology Head & Neck Surgery , Peking University Ninth School of Clinical Medicine, Beijing Shijitan Hospital , Beijng , PR China ;
b Department of Otolaryngology Head & Neck Surgery , Beijing Shijitan Hospital, Capital Medical University , Beijing , PR China ;
Acta Otolaryngol. 2016;136(5):522-7. doi: 10.3109/00016489.2015.1128122. Epub 2016 Jan 27.
Distribution of SP and NK-1R, especially NK-1R, in nasal mucosal tissue at contact point was higher compared with non-contact point. It was suggested that SP and NK-1R were associated with mucosal contact point headache.
To observe difference of substance P (SP) and NK-1 receptor (NK-1R) expression in tissues at contact point and non-contact point among the patients with intranasal mucosal contact point headache and speculate the role of SP and NK-1R in mucosal contact point headache.
SP and NK-1R in tissues of contact point and non-contact point among 40 patients with intranasal mucosal contact point headache were stained histologically by immunohistochemistry, and the mRNA level was detected by RT-PCR.
SP was located in cytoplasm of acini epithelial cells, distributed in nasal mucosa tissues at both contact point and non-contact point. However, stain intensity was significantly increased at contact point (Z = -2.554, p < 0.05). NK-1R was located in the cytoplasm of acinar epithelial cells, inflammatory cells, and nerve fibers of contact point; only in cytoplasm of acinar epithelial cells and nerve fibers of non-contact point. Tinctorial rate of NK-1R increased significantly at contact point (χ(2) = 40.438, p < 0.01). mRNA level of SP and NK-1R was up-regulated in nasal mucosa at contact point compared with non-contact point.
与非接触点相比，鼻黏膜组织中接触点处的P物质（SP）和神经激肽-1受体（NK-1R），尤其是NK-1R的分布更高。提示SP和NK-1R与黏膜接触点性头痛有关。
观察鼻黏膜接触点性头痛患者接触点和非接触点组织中P物质（SP）和NK-1受体（NK-1R）表达的差异，并推测SP和NK-1R在黏膜接触点性头痛中的作用。
对40例鼻黏膜接触点性头痛患者接触点和非接触点组织中的SP和NK-1R进行免疫组织化学染色，并采用逆转录聚合酶链反应（RT-PCR）检测mRNA水平。
SP位于腺泡上皮细胞的细胞质中，在接触点和非接触点的鼻黏膜组织中均有分布。然而，接触点处的染色强度显著增加（Z = -2.554，p < 0.05）。NK-1R位于接触点的腺泡上皮细胞、炎性细胞和神经纤维的细胞质中；仅位于非接触点的腺泡上皮细胞和神经纤维的细胞质中。接触点处NK-1R的染色率显著增加（χ(2) = 40.438，p < 0.01）。与非接触点相比，接触点鼻黏膜中SP和NK-1R的mRNA水平上调。
