Sacan Ozlem, Turkyilmaz Ismet Burcu, Bayrak Bertan Boran, Mutlu Ozgur, Akev Nuriye, Yanardag Refiye
Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34116, Istanbul, Turkey.
Biometals. 2016 Apr;29(2):239-48. doi: 10.1007/s10534-016-9911-y. Epub 2016 Jan 27.
Zinc (Zn) is a component of numerous enzymes that function in a wide range of biological process, including growth, development, immunity and intermediary metabolism. Zn may play a role in chronic states such as cardiovascular disease and diabetes mellitus. Zn acts as cofactor and for many enzymes and proteins and has antioxidant, antiinflammatory and antiapoptotic effects. Taking into consideration that lung is a possible target organ for diabetic complications, the aim of this study was to investigate the protective role of zinc on the glycoprotein content and antioxidant enzyme activities of streptozotocin (STZ) induced diabetic rat tissues. Female Swiss albino rats were divided into four groups. Group I, control; Group II, control + zinc sulfate; Group III, STZ-diabetic; Group IV, diabetic + zinc sulfate. Diabetes was induced by intraperitoneal injection of STZ (65 mg/kg body weight). Zinc sulfate was given daily by gavage at a dose of 100 mg/kg body weight every day for 60 days to groups II and IV. At the last day of the experiment, rats were sacrificed, lung tissues were taken. Also, glycoprotein components, tissue factor (TF) activity, protein carbonyl (PC), advanced oxidative protein products (AOPP), hydroxyproline, and enzyme activities in lung tissues were determined. Glycoprotein components, TF activity, lipid peroxidation, non enzymatic glycation, PC, AOPP, hydroxyl proline, lactate dehydrogenase, catalase, superoxide dismutase, myeloperoxidase, xanthine oxidase, adenosine deaminase and prolidase significantly increased in lung tissues of diabetic rats. Also, glutathione levels, paraoxonase, arylesterase, carbonic anhydrase, and Na(+)/K(+)- ATPase activities were decreased. Administration of zinc significantly reversed these effects. Thus, the study indicates that zinc possesses a significantly beneficial effect on the glycoprotein components and oxidant/antioxidant enzyme activities.
锌（Zn）是众多酶的组成成分，这些酶在广泛的生物过程中发挥作用，包括生长、发育、免疫和中间代谢。锌可能在诸如心血管疾病和糖尿病等慢性疾病状态中发挥作用。锌作为许多酶和蛋白质的辅助因子，具有抗氧化、抗炎和抗凋亡作用。鉴于肺是糖尿病并发症的一个可能靶器官，本研究的目的是探讨锌对链脲佐菌素（STZ）诱导的糖尿病大鼠组织中糖蛋白含量和抗氧化酶活性的保护作用。雌性瑞士白化大鼠被分为四组。第一组，对照组；第二组，对照 + 硫酸锌；第三组，STZ诱导的糖尿病组；第四组，糖尿病 + 硫酸锌。通过腹腔注射STZ（65 mg/kg体重）诱导糖尿病。给第二组和第四组大鼠每天经口灌胃给予剂量为100 mg/kg体重的硫酸锌，持续60天。在实验的最后一天，处死大鼠，取肺组织。此外，测定肺组织中的糖蛋白成分、组织因子（TF）活性、蛋白质羰基（PC）、晚期氧化蛋白产物（AOPP）、羟脯氨酸以及酶活性。糖尿病大鼠肺组织中的糖蛋白成分、TF活性、脂质过氧化、非酶糖基化、PC、AOPP、羟脯氨酸、乳酸脱氢酶、过氧化氢酶、超氧化物歧化酶、髓过氧化物酶、黄嘌呤氧化酶、腺苷脱氨酶和脯氨酰肽酶显著增加。此外，谷胱甘肽水平、对氧磷酶、芳基酯酶、碳酸酐酶以及Na(+)/K(+)-ATP酶活性降低。锌的给药显著逆转了这些效应。因此，该研究表明锌对糖蛋白成分和氧化/抗氧化酶活性具有显著的有益作用。
