Zhang Chengjun, Yang Hong, Yang Huizhao
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, People's Republic of China.
Bioinform Biol Insights. 2016 Jan 20;9(Suppl 1):47-52. doi: 10.4137/BBI.S33716. eCollection 2015.
Alternative splicing (AS) is one of the most important ways to enhance the functional diversity of genes. Huge amounts of data have been produced by microarray, expressed sequence tag, and RNA-seq, and plenty of methods have been developed specifically for this task. The most frequently asked questions in previous research were as follows. What is the content rate of AS genes among the whole gene set? How many AS types are presented in the genome, and which type is dominant? How about the conservation ability of AS among different species? Which kinds of isoforms from some genes have the environmental response to help individual adaptation? Based on this background, we collected analysis results from 17 species to try to map out the landscape of AS studies in plants. We have noted the shortages of previous results, and we appeal to all scientists working in the AS field to make a standard protocol so that analyses between different projects are comparable.
可变剪接(Alternative splicing,AS)是增强基因功能多样性的最重要方式之一。微阵列、表达序列标签和RNA测序已产生了大量数据,并且专门针对此任务开发了许多方法。以往研究中最常被问到的问题如下。在整个基因集中,AS基因的含量比例是多少?基因组中呈现出多少种AS类型,哪种类型占主导?不同物种间AS的保守能力如何?某些基因的哪些异构体具有环境响应以帮助个体适应?基于此背景,我们收集了17个物种的分析结果,试图勾勒出植物AS研究的全景。我们注意到了以往结果的不足之处,呼吁所有从事AS领域研究的科学家制定一个标准方案,以便不同项目之间的分析具有可比性。